Fractions

瀀瀀ract The purpose of Isolation of Bixin from Annatto Seeds using Column Chromatography was to separate and purify macroscopic amounts of pure bixin compound from annatto seed ex-tract.1 A mixture of bixin, methylbixin, and norbixin were extracted from annatto seeds. Bixin was then isolated from the mixture through the use of the column chromatography technique. The isolated bixin was characterized through Thin Layer Chromatography (TLC) analysis, which displayed an Rf value of 0.317, which…

other students work and critique or defend the work. Students will also be able to question another’s work as well as answer questions about their own reasoning. Develop a Productive Mathematical Disposition recognize the importance of decimals, fractions, and geometric patterns as part of their everyday life. View struggle as part of the problem solving process, understanding the importance of perseverance and active engagement. Develop the Ability to Make Conjectures, Model, and Generalize use…

I don’t panic with every single fraction problem but the majority, for example, problem number seven I managed to solve it easily. But with problem number 20, I just couldn’t find the answer, I meant the answer was given to me on the pdf but I still can’t figure out how to solve it. I even tried googling it and I am still confused, I managed to multiply the fraction ¾ by 12 because a foot has 12 inches but I just don’t see how to get 4 1/8 or 33/8…

When comparing this result to those found in Figure 1 from the elution of the blood serum, the profile shown for Iron (III) Chloride is what I would expect. The peak off the elution profile is fraction number 3 which falls in between the highest fraction number of protein which is 2 and the highest fraction number of sodium ions which is 5 according to Figure 1. This can be justified by looking at the molecular weight off all three. The molecular weight of Iron (III) chloride is 162.20 g/mol…

gel indicated that in flow of fraction 1, the band had migrated all the way down the ladder. The multiple bands in the result means it contained many impurities. For fraction 2 with TEA wash, the bands migrated the same length as the first fraction which implies that it also contained impurities. For fraction 3, 5 mM of imidazole was eluted with the solution and it showed a light band at 38 kDa. It means there was presence of proteins in this fraction. For fraction 4, a higher concentration of…

experimental Western blots completed: blot one would contain the ER fraction and blot two would contain the transmembrane fraction. Both blot one and two would be exposed to primary and secondary antibodies (one primary Ab would be specific for MH1 and one primary Ab would be specific for MH2). There would be two control Western blots completed: blot three would contain the ER fraction and blot four would contain the transmembrane fraction. Both blot three and four would not be exposed to…

Each fraction was prepared in an eppendorf tube and the solutions were added using a 100uL-1000uL micropipette and micropipette tips. The first was the blank solution that only contained 800 microliters of distilled water and 200 microliters of the BioRad protein assay reagent. The second fraction was made with 200 microliters of distilled water, 600 microliters of the BioRad protein assay reagent, and 200 microliters of Bovine serum albumin standard solution (BSA). The following fractions had…

By collecting the distillate at each temperature plateau, fractions containing a single component can be obtained. However, this technique does not work for solutions in which the boiling points of the components differ by less than about 40°C. In such cases, a plot of the temperature versus volume for a simple…

4퐶푂퐶퐻푂퐶퐻푂12푋12 = 1442푋2 = 2422푋1 = 22 6푋1 = 611푋16 = 176 1푋16 = 16144 + 22 + 176 = 342 푔/푚표푙 푠푢푐푟표푠푒24 + 6 + 16 = 46 푔/푚표푙 푒푡ℎ푎푛표푙(2.0푔 푠푢푐푟표푠푒)(Table 2. Weight of Ethanol Product and Percent Yield of Fermentation ReactionWeight of Fraction 10.309 gWeight of Fraction 20.344 gPercent Yield45%Calculations:Mass of Vial 1Mass of Vial 2Mass of distillate 1Volume of distillate 1Mass of distillate 2Volume of distillate 2Density of distillate 1Density of distillate 2Percent…

by the decreased absorbance by the Spec 20 over time can be viewed in Figure 1. The total change in absorbance can be seen in Figure 2. The line representing no cell fraction was a negative control in which little to no change was expected. The independent variable was the cell fraction added to solution, and the dependent fraction was the resulting absorbance decrease. The solution with supernatant 3 has the smallest change in absorption besides the control, and the solution containing pellet 3…