Folic acid

Experimental Design Aim 1: Determine the amino acid sensing specificity of folliculin. Since folliculin’s novel amino acid sensing function discovery, I plan to further understand whether FLCN specifically senses a certain amino acid and if this level of detection is enough to elicit an mTORC1 response. 1.1. Determine if folliculin’s novel molecular function in amino acid sensing is triggered by glutamine. mTORC1 activation by amino acids requires FLCN where its lysosomal residence during…

Section Two - Oral Liquid Formulations (1) Characteristics of Suspensions: The oral liquid dosage form chosen to formulate was that of a suspension. Given the criteria of Drug Y it was thought best to formulate the drug as a suspension. Suspensions can be defined as ''a class of materials in which one phase, a solid, is dispersed in a second phase, generally a liquid.'' (1) There are many characteristics of suspensions that are relevant when formulating such a dosage form. They are as follows;…

(Representing the results obtained from the experiment) Known Acid HCl 1 mol dm-3 (± 0.100) Unknown Acid HCl 0.76 mol dm-3 (± 0.100) Highest Temperature (Time) 28.5 (190s) 28.6 (235s) Lowest Temperature (Time) 22.9 (55s) 22.9 (55s) Sample Calculation for the Unknown Concentration C1V1 = C2V2 1 (190 - 505) = C2 (235 - 505) 1 28.0 = C2 37.0 C2 = 2837 C2 = 0.760 mol dm-3 (±0.0500) Sample Calculation for the amount of mole in Unknown Acid Mole = Concentration Volume Mole = 0.760…

as central nervous system stimulant (British Pharmacopoeia 2009). • Methyl paraben was generously donated by Arab Pharmaceutical Manufacturing Company. Methyl paraben is methyl-4-hydroxybenzoate, prepared by the esterification of p-hydroxybenzoic acid with methanol. It occurs as colorless crystals or a white crystalline powder. It is odorless or almost odorless and has a slight burning taste (Rowe et al., 2009). Methyl paraben is very slightly soluble in water, freely soluble in alcohol and…

a 25-mL round-bottomed flask along with a stir bar. To dissolve the camphene, 3.0 mL of glacial acetic acid was added into the flask. The flask was fastened onto a ring stand and immersed in a water bath that was on top of a stir plate. A thermometer wire was placed in the water bath to monitor the temperature throughout the experiment. After the camphene dissolved, 0.50 mL of 8 M sulfuric acid (H2SO4) was added. To prepare for heating, a cork was placed upside down to cap the mixture and…

9. RESULTS Table 8: Pre Formulation Study S.NO API CHARACTERISATION RESULTS 1 Physical Appearance a white to slightly pink crystalline powder 2 Melting point 138-148 ºC 3 Bulk density 0.396 gm/ml 4 Tapped Density 0.421 gm/ml 5 Carr’s index/Compressibility index 5.94 6 Hausner’s Ratio 1.063 Conclusion: The value of compressibility index above 25%, 15-25%, less than10% indicates poor flowability, optimum flowability and high flowability respectively. As Efaverinz value is <10 so it exhibits…

2.4 IL-DLLME procedures For IL-DLLME under optimum conditions, 10 mL of the sample solution containing Cd2+ or Ni2+ and PAN (10−5mol L−1) was adjusted to pH in a glass test tube with a conical bottom test tube. Using a microsyringe, the mixture of the extraction solvent ([Hmim][PF6]) and disperser solvent (acetonitrile) was rapidly injected into the previously mentioned aqueous sample. A cloudy solution (water, acetonitrile and [Hmim][PF6] ) was formed in the test tube. The ions reacted with…

A simple, economic, selective, precise, accurate and sensitive HPTLC method for analysis of Cilnidipine and Chlorthalidone, as a bulk drug and in formulation, was developed and validated according to ICH guidelines. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase while the solvent consisted of Toluene: Methanol: Ethyl acetate (8:2:0.1 v/v/v). The system was found to give compact spot for drug (Rf value of Cilnidipine and Chlorthalidone was…

Abstract For this experiment the amount of zinc in vitamin water had to be determined. To do this, three methods were applied. The first was by a calibration plot method, where zinc standards were prepared and from the absorbance of those standards a calibration curve was plotted, which in turn was used to determine the amount of zinc in vitamin water. The second method was standard addition. For this method, an un-spiked and spiked solution were prepared. The un-spiked solution only contained…

Also, we used acidic indicator phenolphthalein to determine the end point of experiment when the color turns from colorless into red or pink. Potassium acid phthalate is our primary standard and this huge molecule has molecular mass equal to 204.2 g/mol. (KHC_8 H_4 O_4 ) and it has abbreviation as KHP. Also KHP is monoprotic and will react in a simple one to one ratio: NaOH(aq) + KHC_8 H_4 O_4 (aq) →KNaC_8…