Fluorescence

coli was able to take up DNA (pGLO plasmid) via transformation. It can also be concluded that the ampicillin resistance gene was transferred (form of growth) and the gfp gene was phenotypically expressed (fluorescence from UV light) in the LB/amp/ara medium containing the pGLO+ culture. Overall, the results from the pGLO- and pGLO+ transformations seem to be accurate. However, the results are subject to imperfections due to evenly spreading the transfer DNA…

Nano-dots in Mammalian Gametes Imagining1 The Nano-dots, or Quantum Dots (QD), are nano-scale semiconductor devices with diameter up to 10nm, which were first discovered in 1980s2. Once stimulated, they give emission of light with specific colours, which is then visualised1-3. These nano particles have high size-tunability, once illuminated, different emission wavelength can be obtained by manipulating the size of dots3. Inorganic QDs are made up of two components, an atom-cluster core with…

found that extracts of yeast, liver, or rice bran that did not have thaimine did prevented the growth failure in rats that were fed a thiamin supplemented diet. Further, they noted that the yellow-green fluorescence extract promoted rat growth, and they notcied that the brightness of fluorescence was proportional to the effect on growth rate. This resulted in them being to create a rapid chemical that isolates the factor from egg white. they named this…

The transmission of signals across neurons relies on proper release of neurotransmitters. Neurotransmitters are released from synaptic vesicles after they move down the axon and fuse across the cleft. This vesicle transport depends on the protein kinesin which moves synaptic vesicles down the axon to the synapse for release. The inability to move a vesicle down the axon will be kinesin related since kinesin is linked principally with organelles that travel in the anterograde direction on the…

fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci U S A 2001; 98: 3197–3202. 3 Akerboom J, Rivera JDV, Rodr??guez Guilbe MM, Malav?? ECA, Hernandez HH, Tian L et al. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Biol Chem 2009; 284:…

ethanol soluble fraction of V. myrtillus was more than double than from V. uliginosum. 6.4.1. Fluorescence of various fractions of V. macrocarpon, V. myrtillus and V. uliginosum Fluorescence analysis is an important phenomenon exhibited by various chemical constituents present in plant material. Some crude drugs are often assessed qualitatively based on the fluorescent colour. The fluorescence study of different fractions of V. macrocarpon, V. myrtillus and V. uliginosum,…

Lymphoedema is a severe and chronic swelling triggered by a problem in the lymphatic system. It could be produced by a genetic abnormality due to underdevelopment of the lymphatic system or secondary to external factors as infection, injury or cancer treatment [1]. Being breast cancer the 2nd most diagnosed from overall cancers, with patients developing lymphoedema in 40% of the cases [2]. Lymphedema is caused by the inefficiency of the lymphatics to drain fluids and proteins, developing an…

2.7. Hippocampal mitochondrial function 2.7.1. Hippocampal mitochondrial isolation After MWM test was finished, all animals were sacrificed and hippocampal tissues were removed. These tissues were minced and homogenized with glass handheld homogenizer. Mitochondria were obtained from the rat's hippocampus by differential centrifugation [38]. In the first centrifugation (at 1500 g, 10 min at 4°C), all of the broken cell debris and nuclei were sedimented. Then the supernatant was centrifuged…

antibody to be able to detect the enzyme attached to the bound antibody through a substrate. This substrate is what causes a color change, fluorescence or luminescence.…

calculated as k(F)= P(F)/Δt. 5.3.4 Chemical Denaturation Chemical denaturation experiments were carried out on a Cary Eclipse fluorescence spectrophotometer. The concentration of protein in fluorescence measurement was 0.2 mg/mL. The tryptophan fluorescence of protein at 350 nm was monitored at different concentration of guanidine hydrochloride. The result of fluorescence is fitted using the following equation: f(D) = ((aN+bN×D)+(aD+bD×D) ×exp(m× (D-D1/2)/0.592))/(1+exp(m× (D-D1/2)/0.592))…