Fluorescence

In order to determine the sequence of events that occurred at a crime scene it is important to be able to determine and identify certain bodily fluids versus non-human substances. Based on the location of the biological evidence and its DNA can provide crucial information about the crime scene, its victim, and the suspect(s). These tests divided between presumptive and confirmatory testing. Blood evidence is the most common type bodily fluid found at a crime scene. Presumptive testing at the…

FlowMAP is a microfluidic platform developed to measure the overall hemostatic phenotype of an individual, coagulation profile. It is willing to replace the current tests of thrombogenecity, that are focused on isolated components of clotting and under standardized conditions. These individual’s hemostatic profiles under certain conditions and particular drugs will lead to a better understanding of bleeding risk ad clinical clotting prediction. Unlike other complex bench-top tests, it can…

to faster and brighter versions, denoted QuasAr2. In parallel, we utilized the principle of fluorescent resonance energy transfer (FRET) and developed a palette bright eFRET voltage indicators using the donor fluorescence of various fluorescent proteins, rather than the acceptor fluorescence of dim Arch indicator, as readout of membrane potential3. To date, the aforementioned optogenetic indicators have been distributed to and used by hundreds of research labs worldwide. For theme 2, I have…

excitation source to create excited states promoting fluorescence, which we will detect using a photomultiplier tube (PMT). The fluorescence is an expression of mCherry, which gets activated when cells respond to several environmental factors like QS signals or oxygen levels that promote changes in gene expression. For instance, if bacteria are participating in QS, then the genes controlled by QS will be expressed, consequently turning fluorescence on. Thus, we will use the activation of…

blue staining test, shown in table 1 and graph 1, were calculated using formula 1 and formula 2. The results from graph 1 and table 1 show that the untreated cells have the best viability, at 97.5%. Additionally, the results from the differential fluorescence staining and the gelatin zymography are limited because the cells treated with 10% vitamin D3 and the cells treated with 30% vitamin D3 and retinoic acid both had low viability, with 78.6% and 70% respectively. The cells treated with 10%…

The kinetics can be captured at a quick rate of change in fluorescence in order to verify accuracy when compared to real time voltage change. The waveforms generated mimic potential changes distinctly whereas ArcLight lacks the specificity. ASAP 1 can be localized to one specific area, on the membrane to gain the…

Venous blood samples (~5 ml) were collected from normal healthy subjects (n-10) and patients presenting with NSTEMI (n=50) on admission. Based on the duration between the onset of chest pain symptoms and hospital admission, samples were divided into three groups such as hospital admission within 1 hour ( ≤1 hr), or after 1 hour but within 4 hours ( ≥1 hr -≤4 hrs) and after 4 hours but within 8 hours (≥4 hrs- ≤8 hrs) after the onset of chest pain. Half of the blood sample was allowed to stand…

1a. The results from Figure 1 illustrate that the control treatment of the Tomato plant, has a maximum quantum yield of Photosystem II than chilled plants. It appears that over time, the average Fv/Fm seems to be constant with no drastic changes in the levels, with values staying between the ranges of 0.7 to 0.8 Fv/Fm. On the other hand, the chilled treatment of Tomato plants begins at 0.2257 Fv/Fm at time = 0 minutes, and the last plant has a Fv/Fm value of 0.6526 when time is at 150 minutes.…

In Tooth Decay Battle, Laser for the Win! [long form] As Darth Vader knows, a laser is a valuable aid in taking out your enemies. It turns out our Alma, MI dentist Dr. Thomas J. McDonald has a little something in common with the “Star Wars” bad guy. Like the Sith Lord, Dr. McDonald frequently arms himself with a laser on the job. Unlike Vader, though, he uses his laser for good, not evil. Dr. McDonald uses his laser in the battle against tooth decay, every dentist’s sworn enemy. He uses the…

an incredibly useful tool in analyzing the structure and composition of compounds. Specifically, the principles of absorption, the process by which an electron absorbs radiation to excite from a lower energy state to a higher energy state, and fluorescence, the emission of photons when a material is subjected to radiation, allow for the identification and analysis of unknown concentrations of compounds.1 With that being said, the purpose of this experiment is to spectroscopically assess the…