Fluorescence

Exploring Bacterial Transformation Ian Nall BIO 181 | Section #73116 | Wednesday 1:30-4:15pm Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed…

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

Then observed after staining with DAPI (4, 6-diamino-2phenylindole-2HCl, a fluorescent dye) which gives strong fluorescence when bounds DNA (Wicks BL et al, 1996). The other method for observantion of septa and DNA i. e. Calcofluor white-Sytox Green staining method wherer the slide washed three times with PBSand then fixed by using 1% Triton 100X in PBS. The agar section…

Introduction In our society, people may think Mars would be easier to understand. Similiar to Earth, Mars may has ice and water in its atmosphere, different wheather in seasonal, and other recognizable features. However, conditions on Mars vary wildly from what people know on our own planet.Solar system has influenced numerous cultures effecting legends, religious beliefs and at the beginning of the 21st century. Mars Odyssey have long been achieved which is a part of Mars Exploration…

Figure 1: Shows the gene expression changes found in Arabidopsis after the insertion of the IRX1, IRX3, and IRX5 mutants(Brown). 1A shows the principal component data collected from the targeted areas of the plant(Brown). 1B shows a comparison of gene expression of the base and tip of the stem and the change of level(Brown). 1C shows the slope of genes most likely to be related to IRX3(Brown). From Figure 1B, it is shown through analysis that there are several hundred genes that show an increase…

1. Introduction Silicified wood is normally formed close to the surface due to the infiltration of buried wood with mineral-rich water and the associated deposition of minerals (Kim et al., 2009). Two typical geological environments have been reported, 1) trees transported by streams and rivers then buried in the fluvial sediments (e.g., Weibel, 1996; Fielding and Alexander, 2001; Parrish and Falcon-Lang, 2007; Hartmann et al., 2010), and 2) ash from volcanic eruptions buries trees while felled…

The Effects of Technology on Biomedicine Dontae Weatherly CRN: Submitted [date and time] The Effects of Technology on Biomedicine INTRODUCTION Biomedicine is a branch of medical sciences that ties biology and clinical practice together. Biomedicine or medical biology is a major facet of modern health care and lab diagnostics. Biomedicine and biotechnology go hand in hand, with that being said, technology has a great impact on the advancement of biomedicine.…

Doctors and scientist use blood every day for research. For examples, they use blood in studies to help make medication or to even find cures. Doctors also use their patients’ blood to preform test to see if they have some sort of an affection or life-threating virus that is causing their patients to be sick. Reasons why doctors use blood for their test or research is because the human blood or any organisms blood is very complex because it has all sorts of pathogens, leukocytes (white blood…

Hodgkins Lyphoma According to the American Cancer Society more than 8,500 americans are diagnosed with Hodgkins Lymphoma each year occurring in people aged between 16 and 34 years of age and those over the age of 55. Lymphoma is a term defined as a type of cancer that develops in the lymphatic system which is part of our immune system. Hodgkins Lymphoma starts off by first developing in the white blood cells, developing in the lymph nodes and then spreading to any organ which includes the skin…

which has a blue consistency. Conversely, Resofurin is a highly fluorescent red colored compound. When the components interact, viable cells are actively involved in the conversion of resazurin to resofurin. The event triggers an increase in the fluorescence and the color of the media surrounded…