Fluorescence

DEVELOPMENT OF PEANUT ALLERGY Peanut allergies are one of the most prevalent food allergies, along with egg, milk, tree nut, fish, shellfish, wheat and soy [8a]. The exact cause of the development of peanut allergies is still unknown. Studies have showed a positive correlation between mothers eating peanuts during pregnancy and lowered occurrence of peanut allergy in the offspring [1,2]. However, this is contradicted by studies that found no apparent effect of maternal peanut consumption on…

Below I am discussing, at length why I believe I should receive the Research Plus Award, addressing each point of the Researcher Excellence Framework, giving evidence how the past two and a half years have contributed to my personal and my career development. 1. Knowledge and Intellectual abilities a. Knowledge base Throughout my PhD I have been able to attend a number of courses organised by the QUB postgraduate centre, which allowed me to further my knowledge and gain valuable skills within my…

reefs from large-scale benthic survey images. The paper reported that reflectance images itself won’t always contain sufficient information for proper classification of coral reefs, and further suggested to combine those reflectance images with fluorescence images to obtain more accurate results. This discovery is spectacular for its originality, utility, and extendibility relatively to other ecological applications. As my intense interest in computer vision was introduced earlier, I am…

Bacterial Transformation with Escherichia coli Byanka Delgado BIO 181 Lab Thursday 1:30-4:15 Arizona State University Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates,…

Genome-Wide Mapping of in Vivo Protein-DNA Interactions Vast knowledge is available on transcription factor binding. Unfortunately, not much is known about the structure and function of complete DNA interactomes. Even less is known about organisms with larger genomes. Presently mouse, human, and other large genomes have been sequenced. Thus, it is feasible to quantify how transcription factors are carried along a whole genome for various cells in physiological conditions. If measurements are…

C. APPROACH: C.1. Experimental rigor: We will apply our established in vitro and in vivo analyses to the studies proposed in both aims (31, 41, 42). Our preliminary data demonstrated the vigorous infection in both Heterozygotes (Foxn1nu/+) and homozygotes (Foxn1nu/nu) NU/J mice at mucosal sites. To minimize the possible variations in the animals, we will use siblings of these two inbred mouse strains. For the experiments proposed in both specific aims, we will use fifteen animals in each group…

Methods Each trial consisted of isolating the hearts of two mice, aged anywhere between 8-15 weeks, with the strain FVB/NJ of the Mus genus. Eight trials were conducted throughout the duration of the experiment. No female mice were used for any of the trials because because the research in the lab concentrates on males, due to known differences in protein expression among the two sexes. The effect of the VU591 drug on mitochondrial KATP function in wildtype mice was measured by calculating the…

THP-1 cells and THP-1 cell culture methodology were also provided by Ondek Pty Ltd. THP-1 cells were cultured in RPMI-10 culture medium. The first THP-1 cell culture was made by Dr Senta Walton, with a starting concentration of 2 x 105 cells/mL. The cell culture was then incubated at 37°C with 5% CO2 and subcultured every 2-3 days, when the cell concentration reached approximately 8 x 105 cells/mL. THP-1 cell based assay THP-1 cell based assay was performed to determine the immune modulatory…

1-pyrenehexanoic acid was used as the 5-carbon linker and fluorescent component of the target molecule "PYRET-5". This molecule was purchased pure along with the sugar Trehalose, the molecule to which the pyrene was to be attached. Other reactants and solvents include HATU, HBTU, N, N-Diisopropylethylamine (DIPEA), sodium azide, Dimethylformamide (DMF), and Diphenyl phosphoryl chloride and were bought pure or used as received. All reactions were run at room temperature (298.15 K) and standard…

Joshua K. Westervelt Dr. Fair MCB 4404 11-13-17 Scientific Article Analysis Questionnaire A. Read the abstract to get an overview of the article. B. Read the entire article and write notes from each section (Introduction/ Materials-Methods/ Results/ Discussion). Always refer back to the abstract if you get lost in the main sections of the article. C. Then answer following questions (also see “Explanation of Article Sections” below). ABSTRACT/OVERVIEW QUESTIONS: 1. Who did the research, and…