Fluorescence

It has been demonstrated that MUC1 (membrane bound mucin) often located on the outer portions of cells, serves as a preventive agent against Helicobacter pylori. MUC1 reduces gastric inflammation in H. pylori Infected tissue by forming a complex with IkB kinase gamma (IKKγ) in this way hindering the contact between IKKγ and IKKβ causing the prevention of nuclear factor kappa B (NF-kB) transportation to the nucleus, in this manner preventing the transcription of genes that can cause inflammation…

Circular Dichroism (CD) fundamentals. CD is defined as the difference between absorptions of left (Al) and right (Ar) circularly polarized light beams, CD=Al-Ar For each absorption band, the CD of two enantiomers are always exactly opposite. Theory. For each electronic transition one can define an electric (µij≠0) and a magnetic (mij≠0) transition dipole. Both can lead to absorption of radiation, the intensity of which is directly related to the oscillator strength f, fij≈| µij |2+ | mij |2 In…

Two chromatographic methods were developed for the assay of the FDA approved lozenges containing dextromethorphan hydrobromide (DXT) and menthol (MNT). The first was a green HPTLC method which depends on using Merck HPTLC aluminum sheets of silica gel 60 F254 and methanol-ammonia (10:0.1, v/v) as mobile phase. This was followed by densitometric measurements of the spots which were retained at 0.28 ± 0.01 for DXT and 0.76 ± 0.02 for MNT scanned at 210 nm. The method was found linear over the…

Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…

Figure 2.1.3 TPGS concentration and Paclitaxel solubility are directly related. Figure created by M. Varma, 200522. TPGS has been incorporated into various drug formulations partly because of its ability to overcome multidrug resistance (MDR). By inhibiting P-glycoprotein (P-gp) activity, the relative cytotoxicity of drugs such as doxorubicin, vinblastine, and paclitaxel was increased19. P-gp is a drug efflux pump, a.k.a. multidrug resistance protein 1 (MDR1), located in the cell membranes of…

The Basso, Beattie, and Bresnahan (BBB) locomotor scale The Basso, Beattie, and Bresnahan (BBB) scale (numbering 0–21) is a point scale used in the analyzation of the activity of the limb joints of animals. Its locomotion rate is based on various aspects of the hind limb function like the weight support and toe clearance, just to mention a few [37]. All results were obtained at time points of 2 weeks to 8 weeks post-operation along with the blinded examination that was executed by two scorers.…

Human epidermal receptor 2 positive (HER2+) Breast-Cancer (Bc) is one of the most aggressive subtypes of Breast-Cancers that is found in approximately 20% of them (1,4). HER2+BC remains a major cause of morbidity and mortality in women all over the world as once the advanced or metastatic HER2+BC is developed, no treatment can stop the progression. The combination of Trastuzumab; a monoclonal antibody that blocks HER2 receptors, and Docetaxel; a cytotoxic agent, has been approved for use as…

Murphy wrote, “And I don’t remember if I actually witnessed- or just imagined- Connie and her mother at the 7-Eleven one day after school. They were in the candy aisle. Her mother was filling a cardboard box. And Connie, bathed in unflinching fluorescence, was curved over her notebook making small, careful checks” (155). The route that Connie’s mother takes with handling this situation displays the actions of a true parent. Although her daughter is being picked…

In this study, the effect of benzenethiol ligand on CdSe/ZnS core/shell quantum dots was investigated. Conventional long-chain oleic acid (OA) and trioctylphosphine (TOP) capping ligands were partially replaced by short benzenethiol ligands, and the improvement in the luminescence performance was observed in quantum dot light emitting diodes (QD-LED). Approximately 1.8 fold improvement was achieved for both the maximum luminance and maximum current efficiency for the QD-LEDs fabricated with…

The instrumentation of a HPLC apparatus is as shown in the diagram above. A HPLC apparatus is including a solvent reservoir, degasser, pump, injector, HPLC column, detector and data collection device. Solvent reservoir The solvent reservoir is usually a glassware or reagent bottle which contained the analytic sample. It is located at the upper part of the HPLC. It is also known as mobile phase reservoir since the sample will be moved. The flowing of the sample is depends on gravitational…