Fluorescence

specifically to antigens in biological tissues through CD3 T-cells. Using immunohistochemistry would make it easy to detect where on the cell surface TLR23 is introduced to the pathogen. The anticipated results would be that when observing the fluorescence of the TLRs that they are found mostly within and around the cell…

There are thousands of diseases in the world. Some diseases can be cured others cant be. Some of these diseases however, deal with genetics. Genetic disease is defined as a disease caused by an abnormality in an individual's genome ("Genetic Disease"). One genetic disease that affects many people is Acute Myeloid Leukemia. Roughly 20,000 people each year are diagnosed with Acute Myeloid Leukemia or AML ("How Many People”). One casualty of these disease is my grandma who passed away of AML in…

from testing a cell. There has been biosensors developed to preform Non-cellular assays. One such sensor, called The Endotect, uses “a human estrogen receptor connected to a fluorescent molecule” (H.S Changa et.al 2009), in such a manner that any fluorescence generated from interaction between the EDC’S and the receptor can be measured. The biosensors are portable and generally faster than the other methods at detecting the EDC’S. One of the drawbacks though is that the sensors generally measure…

The topic of this report is going to be on the ringworm. A ringworm is a fungal infection that occurs on the top layer of the skin, known as the epidermis. They are distinguished by having rough reddened patches that form in circular designs or pattern, and can be spread from your face, all the way to the toes. The ringworm, also called dermatophytosis, is mainly caused by having direct and or indirect contact with a person, animal or object that is already infected with the disease, or one of…

genetically engineered mouse models of CCA, and patient derived xenografts of CCA (PDX). She will also become adept at cell biology approaches including confocal microscopy coupled with computer-based 3-D image reconstruction, expression and use of fluorescence-based bioprobes, gene silencing and editing technology, immunofluorescence and immunohistochemical approaches, and culture of human CCA organoids. She will also become familiar and adept at pursuing biochemical and genetic techniques…

Detection of Zika virus Introduction: Zika Virus, also referred to as ZIKV, has become a global health epidemic. The World Health Organization declared a Public Health Emergency of International Concern earlier this year due to the increase of areas affected by Zika. Originating in the Zika forest of Uganda, ZIKV was predominantly found in areas of Africa and Asia. However, there have recently been reports of more than 36 countries with cases of the virus. (Chen, 1), (CDC) ZIKV is a single…

In this research paper, the functional relationship between protein named Albert and Alice will be prove using two advance techniques. Most of the molecular processes within the cell are carried out by protein-protein interaction (PPI), diseases such as cancer, Alzheimer’s disease can caused by abnormal PPI. As prior knowledge has advocate that this two proteins may have similar cellular pathway, therefore understanding if they have PPI becomes vital. There will be two methods described in this…

DNase and RNase free water (SinaClon BioScience Co, Iran). The PCR condition was: 95 °C for10 min, 45 cycles at 95 °C for 30 s, 63 °C for 30s and 72 °C for 30 s. Then, 95 °C for 1 min and 40 °C for 1 min, followed by HRM ramping from 70–95 °C with fluorescence data acquisition at 0.05 °C increments. The spa types t030, t459 and t016100 were used as controls in HRM experiment. The melting curves of all samples were analyzed by High Resolution Melt Software v3.0(Thermo Fisher ScientificCo,…

Task 1A Tanya Gold’s message is that sexism and misogyny is alive and well even today. By her clever use of sarcasm and comparisons, she manages to give an accurate portrayal of modern society, which holds men and women to different standards. With this text, Gold wishes to raise awareness for prejudice against women and warn the public about the dangers of stereotyping and generalisation. Task 1B In her text, Tanya Gold uses several different literary devices and language features to get…

member’s emails. To examine the results from the DRP1 gene inhibition, the cells had to be injected with mito-GFP. Mito-GFP is a green fluoresces gene from jellyfish. The GFP insertion allowed us to examine the mitochondria in the cells with a fluorescence microscope. This microscope takes three versions of the image and we took three different images from both the dsRNA DRP1 and controlled samples. The images were saved onto the computer and uploaded onto the lab’s Blackboard…