Fluorescence microscope

structure where excitatory neurons are typically pyramidal cells with one apical dendrite and multiple basal dendrites (Megias et al., 2001). The significant structures highlighted above would be examined and identified in the experiment using a light microscope and prepared slides of dog spinal cord, rat cerebral cortex, brain and…

• Bacteria, are single-cell microorganism with no nucleus that had a sphere, rod, and spiral shape and branching threads. Ranges in diameter between (0.5- 1) µm and in length between (2-4) µm however, they are either a parasite or live independently they are found in all living things and in all environments. Some bacteria in drinking water can cause disease such as salmonella, total coliform (including fecal coliform and E.coli), Shigella, Legionella, and Campylobacter and “their presence…

dehydrated in xylene before being mounted with Neutral Balsam. Five transverse sections from each animal were picked out and 3 vision fields randomly selected per section. They were then photographed under 20X magnification using the Nikon TE-300 microscope. The number of neuronal cells per field was counted on the anterior horns as well as the neurons with a well defined nuclear and cell body rich in endoplasmic…

because of its wide range of uses. People use some form fluorite everyday. In industry, fluorite is used as a flux for smelting (University of Minnesota.) The clearest form of fluorite can be used to make lenses. Most commonly used for lenses in microscopes. Each colored fluorite has its own name.…

We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…

presence of the smaller phytoplankton in the community. This allowed us to determine the dominant size of phytoplankton at each station. Chlorophyll a concentration was analyzed by extracting chlorophyll a from the respective filter and measuring fluorescence present in the sample. The filters were placed in centrifuge tubes with 90% acetone and placed into a freezer overnight. The next day, 1 mL of the sample was measured by a fluorometer. After each initial reading, the cuvette was removed…

Kishan Patel Section: 008 Pattern: Calcium levels in the soil controls soil pH and species richness within a community Hypothesis 1: Calcium levels in the soil controls soil pH. Prediction: An increase in the calcium levels in the soil will make the soil less acidic. Reasoning: Calcium exists mainly in the soil as exchangeable cations (ca^2+) associated with basic anions, which reacts with soil acidity (H) to neutralize it. Analytical approach: Regression Independent variable: Calcium levels…

The appropriate description of the clinical appearance often leads to a diagnosis of the illness. • Stained culture/histology examination False coloration of a substance to aid in the inspection of tissues, microorganisms, or other cells under a microscope. • Tests for antibodies to the microorganism Antibody tests rely on on the fact that there are specific antibodies for each antigen and each one can be used to determine the presence of the other. For example, a known antigen can be added…

results (3). In order to better understand the comet assay, its procedure, analysis, application, and limitations will be examined. In order to perform a comet assay, two days are normally needed. On the first day the cell sample is collected and a microscope slide is prepared be embedding the cell in agrose (1,4). The cell is then…

of fluorescent antibody conjugates to the added antibodies; IgG-Alexa Fluor 546, IgM-Alexa Fluor 488, IgG2b-Alexa Fluor 647. The same procedure was performed for the control tissues as well. The slides were imaged under a confocal laser scanning microscope in order to retrieve a cell count of lymphocytes…