Fluorescence microscope

Inflammation is a defense mechanism where the body’s white blood cells fight off bacteria, viruses, and damaged cells(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . When pathogenic cells enter the body, the white blood cells surround that invasion site immediately(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . The white blood cells then release a specific toxic chemical that denatures the pathogen enough to kill it(Feeling the Heat- the Link between…

used a light microscope with an HDMI port attached to a computer. We photographed both controlled and affected samples three times each. The slide images were saved onto the laptop and sent to the group member’s emails. To examine the results from the DRP1 gene inhibition, the cells had to be injected with mito-GFP. Mito-GFP is a green fluoresces gene from jellyfish. The GFP insertion allowed us to examine the mitochondria in the cells with a fluorescence microscope. This microscope takes three…

membrane was tested using Fluorescent Recovery After Photobleaching (FRAP). We used a Nikon Eclipse 50i upright fluorescent microscope with a Digital Sight DM-2MBW CCD camera to take images of the bilayer after photobleaching. Using these images we were able to determine the diffusion coefficients for the bilayer to be , without cholesterol and , with cholesterol. Using the same microscope and camera alongside an orange Helium-Neon Laser at 594 nm we studied the binding of biotin and anti-biotin…

which contained 540 cells in total and lastly well three contained 1,740 cells in total. We weren’t surprised by our results because we had a lot of waiting time in order for the cells to grow. 2. All of the dyes were visible under the fluorescent microscope. The green dye stained the actin, the red dye stained for mitochondria and the blue dye stained for dsDNA. If someone would not be able to see these pictures it would indicate contamination and they would not contain organelles and…

The kinetics can be captured at a quick rate of change in fluorescence in order to verify accuracy when compared to real time voltage change. The waveforms generated mimic potential changes distinctly whereas ArcLight lacks the specificity. ASAP 1 can be localized to one specific area, on the membrane to gain the…

fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci U S A 2001; 98: 3197–3202. 3 Akerboom J, Rivera JDV, Rodr??guez Guilbe MM, Malav?? ECA, Hernandez HH, Tian L et al. Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J Biol Chem 2009; 284:…

“A Novel Application of Microfluidic Assay to Evaluate the Role of Calponin in Platelet Function and Clot Formation” Introduction: Platelets are a very important aspect of blood clotting. They are also essential in the evolution of clots in Coronary and Cerebral arteries, which cause heart attacks and strokes, respectively. Every year, 610,000 people die of heart disease in the United States; this is 1 in every 4 deaths. Cardiovascular disease is the number one cause of death in the United…

certain unusual symptoms which need further diagnoses. Leukemia detection test involves many diagnostic methods such as blood test, bone marrow test, cytogenetic analysis, immunophenotyping, polymerase chain reaction (PCR), immunoglobulin test, fluorescence in situ hybridization (FISH), and G-banding karyotyping. The evaluation of these this diagnostic test takes place in the laboratory to determine the treatment plan for the patient. Most of these diagnostic tests are also in use throughout the…

Gladwell’s excerpt, for instance, he writes, “He then removed a core sample measuring one centimeter in diameter and two centimeters in length from just below the right knee and analyzed it using an electron microscope, electron microprobe, mass spectrometry, X-ray diffraction, and X-ray fluorescence” (1). By listing numerous professional-sounding procedures, Gladwell effectively persuades the reader into thinking that the expert examinations on the work of art is trustworthy, thorough, and…

The first virophage to be discovered is Sputnik virophage. It was found accidentally while examining Mamavirus with an electron microscope. The Acanthamoeba that contained Mamavirus was found in a water cooling tower in Paris. Sputnik depends upon the co-infection of host cells by larger viruses known as Mamavirus and alone is incapable of infecting the host amoeba cell [9]. A versatile…