Fluorescence microscope

(EDS) images and spectra were obtained from various samples of silica and platinum on silica. Each sample contains unique structures which were prepared by etching the surface of silica in different conditions. A scanning electron microscope is a type of electron microscope that produces images of topography and composition by scanning a sample with a focused beam of electrons. Samples can be observed in high vacuum, low vacuum, in wet conditions, or at elevated temperatures. The images in this…

goal was set to “maximize” mode. The optimum parameter which was predicted by the RSM model was then validated experimentally in triplicates. 2.5. Scanning Electron Microscopy analysis of scaffold: The scaffold was examined under scanning electron microscope (SEM) (ZEISS EVO 18) under acceleration voltage of 10 kV. The uncoated sample was used for SEM…

The first scanning electron microscope (SEM) was invented by Manfred von Ardenne in 1937 (McMullan). However, further development of the SEM was accomplished by Charles Oatley and his colleagues at Cambridge University (McMullan; Egerton 17). He and his colleagues acquired their first images from the SEM in 1951 (Egerton 17). The SEM was developed because if a TEM is utilized, a specimen must be made extremely thin to prevent the electrons from dispersing inside the specimen and so the electrons…

Kanamycin sulphate and rifampicin were purchased from Hangzhou Dayangchem Co., Ltd., Zhejiang, China. Acetonitrile and ethanol (High performance liquid chromatography, HPLC, grade) were purchased from Merck, Germany. Sodium dihydrogen phosphate, phenylisocyanate, triethylamine, orthophosphoric acid (analytical reagent grade) and silicone oil (viscosity 10 cSt) were purchased from Sigma–Aldrich, St. Louis, USA. Size 3 hard gelatin capsules were kindly donated by Capsugel Co., Ltd., Tokyo, Japan.…

3.2 Analysis of chip formation Fig. 7 shows the chip forms obtained during milling of the Al7075 (a) and MMCs (b) using the uncoated carbide inserts at a feed rate of 0.1 mm/tooth, machining speed of 290 m/min and axial depth of cut of 1.3 mm. The Al7075 chips formed during milling exhibited a smooth and shiny surface, and were in a semicircular and parabolic trapezoidal wave with a small radius and a saw-tooth-form, as shown in Fig. 7 (a). Fig. 7 (b) shows the chip shapes formed during the…

Since the water splitting reaction using semiconductor electrode reported by Fujishima et al. in 1972 [1], there is increasing interest in the development of metal oxide photocatalysts for environmentally friendly applications such as air purification [2], hydrogen production via water splitting [3] and the removal of organic molecules from water [4]. Recently, photocatalyst thin film has aroused growing interests instead of a powder type of photocatalyst. Photocatalyst film can be deposited…

Technical Review of Hydrothermal Formation of Calcium Copper Tetrasilicate Introduction Scanning electron microscopy (SEM) is used to obtain close-up images of samples with depth enabling high quality, 3D depictions on a microscopic level. It has been historically used to identify samples of interest such as bacterial biofilm, microtexture surfaces, and even cancer cells for diagnosis.[1, 2, 3] The first high magnification SEM was reported in a journal in 1938 by Ardenne after developing the…

3.1.3 FESEM and EDS analysis The structural morphology of the Pure HA, Sr-doped HA, La-doped HA and La/Sr co-doped HA (different La concentrations of 0.03, 0.06 and 0.1M) nanoparticles are analyzed by FESEM images are shown in Fig. 3 (a)-(f). The morphology of pure HA shown in Fig 3 (a) indicates that formation of spherical (or) chain pearl shape of nanoparticles, ranging from 70 to 84nm. Further, Sr (0.1M) doped HA and La (0.1M) doped HA belongs to Fig.3 (b)-(c), appears the formations of few…

Microscopy is one of science’s greatest innovations. The microscope has revolutionised countless areas of scientific research and has become an icon of science itself. From Anton van Leeuwenhoek discovering micro-organisms in the 1670’s to the highly powerful atomic microscopes used today, microscopy has furthered scientific knowledge in many, many ways [1]. It has practical applications in very many areas of research such as biotechnology, materials science, microbiology, environmental science…

rsw1 plants of Arabidopsis at permissive (21°C) and non-permissive temperatures (31°C) were observed under light microscope by staining with different stains which were Toluidine Blue, Ruthenium Red and Calcofluor White. The results are shown below. i. Toluidine Blue Stain Figure 2| Root tip of Col plant at permissive temperature (21°C) stained with Toluidine Blue under light microscope with magnification 4X. The width of the root tip is approximately 0.14 mm. In figure 2, the root cap region…