Fluorescence microscope

light microscope. During this lab you will check the microscopes by drawing what you see to make sure the high and low powers work properly. After you tested your microscope you take a toothpick and scrap it on the side of you mouth and examine what you see through the microscope, your final results should be a drawing of what you saw through both the high and low powered lens. Introduction- This lab is based on the compound light microscope. “A compound light microscope is a microscope…

In exercise 1, experiment A, the students will need a microscope slide, a coverslip, a compound microscope, carmine powder, distilled water, and a pipette. The students will begin by placing a drop of water on the microscope slide. Then they will add carmine powder onto the slide. Then they will place a coverslip on top of the solution. This will aid in mixing the solution. Then the students will observe the movement of the carmine particles on low power and high power. In exercise 1,…

water under the microscope. Taking note of their size, movements, and characteristics to classify the different types of protists from applying previous knowledge of protists. Materials: The materials in this lab that will be needed are a microscope, slide, coverslips, a pipette, and a dish of pond water. Procedure: Prepare a slide by using the pipette to apply two drops of pond water on a slide and properly place a coverslip. Place the prepared slide under the microscope to view and…

the area of the microscope where it can be scanned. Sometimes this means only rather small areas or portions of a sample can be analyzed at one time (1). Another important environmental factor to consider when performing SEM is that atoms in the air can affect the electron behavior too, so the analysis must be done in a vacuum. This prevents certain types of samples from being tested, specifically those in a watery environment or those that are unstable (2). Typical SE microscopes cannot analyze…

Purpose: Observe a compound light microscope to learn the names of its major parts. Research: Microscope is an essential tool for the study of small structures such as cells. The most common type of microscope is the bright-field microscope, used for this purpose is the compound light microscope, it is called compound because it uses two sets of lenses the objective and the ocular. The ocular lens usually magnifies 10x.…

Created by Ernest Ruska and Max Knoll of the Berlin Technische Hochschule in 1931, the invention of this novel microscope allowed for the surpassing of the visible light resolution barrier. Since its inception, the TEM has become a resolution standard in the field of imagery, and further advances have led to the progression of high quality atomic viewing. The development of the electron microscope in the 1930s revolutionized biology, allowing for organelles, such as mitochondria, to be seen in…

Chromium (Cr) is one of the most toxic chemical compounds due to the common use of chromium compounds in industry in addition to its natural sources (Costa et al., 2003). Exposure to chromium occurs via contaminants found in drinking water and food, through air containing particulates or mists of hexavalent chromium Cr (VI) compounds, or through skin contact with soil at hazardous waste sites (National Institute for Occupational Safety and Health, 2013). Cr (VI) may be found in drinking water…

There are many different ways medical technology has progressed over the last one hundred years in Canada. The discovery of insulin helped doctors save many lives. The invention of the electron microscope changed the way scientists were able to view many different things including very small organisms. The invention of the external pacemaker saved many lives back in 1950, one of the inventors needed his own invention to save his life for some time. The discovery of the Cystic Fibrosis also…

sample therefore emits radiant energy that is characteristic of the atoms present (XRF, 2002). Finally, the resulting fluorescent x- ray can be used to get an analysis in ppb on a substance if it is present in the sample (XRF, 2002). The x- ray fluorescence spectrometer in the lab, however, measures in ppm instead of the common ppb. The calculation, therefore, that will be used is 1ppm= 1000ppb. The goals for this senior research project is to determine whether or not there is a high…

Levofloxacin cocrystals were prepared by solvent evaporation method. The drug and co formers, stearic acid and sodium saccharin were physically mixed in varying ratios of 1:1, 1:3, 1:5, 1:7 and 1:1 and 2:1 respectively in a glass mortar and the mixtures were dissolved in ethanol as solvent for crystallization. The solvent evaporation was carried in rotary flash evaporator (Laborota 4000, Hiedolph) maintained at 40 ± 2ºC and speed of 30 rpm. The products obtained were dried in a desiccator and…