Fluorescence microscope

It can be explained as follows; the ultrafast electron pulses with a duration of couple hundreds of femtosecond are generated by the photoemission process by illuminating the photocathode inside the microscope by UV laser pulses. These electron pulses are accelerated inside the microscope and incident on the sample under study together with visible laser pulses. The photon-electron coupling between the optical pulses and the electron pulses takes place when the energy-momentum conservation…

1. How are the photographs taken with a 3D microscope different than those taken with a regular camera? How are they similar? The 3D microscope photographs are used to show detail that a regular photograph could not, for example at a crime scene. The similarity is the basic image, it is the same image but the only difference it has with a 3D microscope is the detail shown. 2. How can photography allow us to view the world around us in different ways? Photography captures not a second of the…

Cellular Theory & Microscopes Robert Hooke Robert Hooke was an English scientist credited most famously for the creation of Hooke’s Law (a principle of physics that states that the force needed to extend or compress a spring by some distance is proportional to that distance) in 1660 and the discovery of plant cells using a compound microscope in 1663. The compound microscope uses two or more lenses, which are connected by a hollow tube. The top lens (called the eyepiece) is the one that people…

A light microscope works like a telescope using glass lenses to reflect light, which magnifies images which are naked to the human eye such as cells and dust. The lens closest to your eye is called the eyepiece lens. This usually has a magnification of x10. Objective lenses are lenses of several different strengths which magnify the image. The stage is the place where the microscopic slide sits. This is held in place by the clips. The light source (can be a mirror or a bulb) projects light onto…

Introduction The microscope is an optical instrument that produces large images of small objects that cannot be observed by the human naked eye. There are two types of light microscopes: compound microscope and Electron microscope. Light microscopes are divided into two categories: Compound microscopes and Stereoscope microscopes. Electron microscope functions in the following way; a stream of electrons emitted from an electron source built in the microscope, the obtained image is in black and…

Discussion Microscope is an optical instrument that have magnifying lens which is used for inspecting objects that is too small to be seen or to be seen distinctly and in details by the unaided eye. Microscope also is an optical instrument that keep observe specimen in resolution and magnitude way. However, there are some microbes such as viruses that cannot be observe by certain microscope and this microbes has to be observe using electronic microscope which has more magnification power. In…

A 12-well tissue culture plate was used to carry out the LIVE/DEAD Assay. Well “A” contained the untreated HepG2 cells. Well “B” contained camptothecin, which is used as a control for killing the HepG2 cells instead of the use of plumbagin. Well “C” contained the compound Epigallocatechin Gallate. Well “D” remained empty, because a vehicle treatment is not needed for this experiment. Each well contained 1 ml of HepG2 cells. A negative and positive control where both set up in the 12-well tissue…

Lab Five: Microscope and Simple Staining Introduction A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a…

Observation of plant and animal cells through a light microscope. A cell is the most basic structure of any living organism and is capable of independently reproducing. Cells can be grouped into two categories, prokaryotic and eukaryotic. In a eukaryotic cell there are small organelles that carry out specific functions which can be compared to the organs in the human body. Some of these organelles include the nucleus, which holds the cells DNA, mitochondria, which produce ATP from glucose and…

The slide used during the Microscope Lab was a wet-mount slide which was used in order to view the specimens from the microscope. A wet-mount is a method for preparing specimens to view using a microscope. A myriad of specimens look better when placed in a drop of water on the microscope slide. The water supports the sample and fills the space between the coverslip and the slide. This allows light to pass easily through the slide, the sample, and the coverslip. Scientists use the wet-mount…