Flow cytometry

facilitated the discovery of new diagnostics and treatments to manage a wide array of diseases. In addition to the above, coupled with advancing technology, immunological research has provided critically important research techniques and tools, such as flow cytometry and antibody…

Photographing microscopic and gross specimens. • Preparing tissue for various pathological tests, including frozen section. • Assisting with the training of pathology personnel: This includes pathology residents. • Immunohistochemical (IHC) staining and flow cytometry. • Preparing educational conference materials for the pathologist. • Procuring samples to be used for biospecimen banking. A pathology assistant may also take on administrative, supervisory and instructional roles. Where PAs…

IC.3. Specific Aims: Specific Aim1: Role of NK cells and neutrophils at different disease stages of MmuPV1 infection Rationale: The role of innate immune cells in HPV-associated cancers has been investigated in human studies (55). However, how these cells fight against papillomavirus infection at different disease stages remains unclear (8). We recently established persistent mucosal infection in immunocompetent heterozygous (Foxn1nu/+) NU/J mice. When compared with immunocompromised homozygous…

24-well plate. After the incubation period, the cells were washed, trypsinized and collected for flow cytometry analysis. The cell suspension was centrifuged at 13,000 g and rinsed with DPBS twice to remove the non-bound NCs, followed by resuspending in 0.1 ml of PBS. The intensity of FITC fluorescence in the cells was detected and cells were counted by flow cytometer (Beckman Coulter Cell Lab Quanta SC flow cytometer, US) equipped with a 488 nm laser to analyze FITC labeled NCs (λex max = 490…

T cells also have effect on Natalizumab in patients with multiple sclerosis. Natalizumab is a monoclonal antibody that reduces the formation of acute lesions in the central nervous system. Bornsen et. al revealed the influence that natalizumab has on circulating CD4+ T cells in multiple sclerosis. The recurrence of multiple sclerosis happens when there are abnormalities in activated macrophages, CD4+ and CD8+ T cells in the central nervous system. Very-late antigen formed on surfaces of most…

It is becoming evident that humans are continuously exposed to a plethora of chemicals from food, medicinal agents, occupational activity and contaminated sites. Exposure to chemicals begins in utero and continues throughout life. Toxicological risk assessment of individual chemicals provides the quantitative estimate of its possible effects on human health. When humans are exposed to chemicals, they are not exposed to a single chemical only, but simultaneous or sequential exposure occurs to a…

Sexed Semen is changing the Face of Cattle Reproduction Worldwide Sexed Sorted Semen is still a relatively new reproductive technology that is impacting cattle producers and semen marketers worldwide. Using flow cytometry, purified sperm is sex sorted into X-bearing chromosome and Y-bearing chromosome populations. These subpopulations can then be frozen and stored for future use in artificial breeding. This technology works well in a well-managed artificial insemination or embryo transfer…

Dr. Joseph Larkin III is an associate professor in the department of Microbiology and Cell Science at the University of Florida where he teaches Immunology. In addition to teaching, Dr. Larkin oversees multiple immunology research projects involving regulatory T cells and their involvement in autoimmune diseases including type one diabetes, lupus, rheumatoid arthritis, and most recently, uveitis. Dr. Larkin was born and raised in Savannah, Georgia. When asked about how he became interested…

Cell culture & cell counting: Introduction: Cell culture is the method of removal of cells from their respective animal or plant source, followed by their growth in a favourable artificial environment. These cells can be removed directly from tissues or fragmented using enzymatic or mechanical method. These cells can be obtained from the already established cell line or cell strain. Short protocol:In this protocol, initially cells were washed to remove adherent cells, incubated and…

NEUROSARCODOSIS PRESENTING AS RAPIDLY PROGRESSIVE DEMENTIA: A CASE REPORT ABSTRACT: We report a case of neurosarcoidosis in a patient presenting with rapidly progressive dementia and intermittent delirium. Sarcoidosis with neurologic involvement is rare, and typically does not manifest with psychiatric or cognitive symptomatology. The most common presentation of neurosarcoidosis is facial neuropathy, followed by meningoencephalitis. This patient exhibited accelerated mental…