Flow cytometry

A flow cytometry based micronucleus test and AhR activation assay were used to assess the effect of PAHs and metals mixtures on genotoxicity and metabolism, respectively. The mixture induced changes in the cell cycle parameters were also assessed. Binary to quaternary…

Bleed all mice (SWP 0082) to obtain day 0 peripheral blood engraftment values by flow cytometry (SWP 0093) 6. Treat mice according to your chosen schedule. For detailed drug administration techniques, see appropriate SWP (SWP 0083/SWP 0084/SWP0087/SWP 0088). NOTE: The mice will need to be closely monitored following treatment as they will…

There are many challenges to successful organ transplants, including matching a recipient and a donor based on matching genetic variability. It is extremely difficult to match MHC perfectly with a donor and a recipient as there are many locations where genetic differences can still cause a rejection reaction by the recipient. A familial donor is usually the closest to a perfect genetic MHC match that a recipient can find. The closer the match of genetic variability, the better the chances that…

Hodgkins Lyphoma According to the American Cancer Society more than 8,500 americans are diagnosed with Hodgkins Lymphoma each year occurring in people aged between 16 and 34 years of age and those over the age of 55. Lymphoma is a term defined as a type of cancer that develops in the lymphatic system which is part of our immune system. Hodgkins Lymphoma starts off by first developing in the white blood cells, developing in the lymph nodes and then spreading to any organ which includes the skin…

III. Plans for Candidates Training and Research Career Development. a. Mentorship Plans to Enhance her Technical Repertoire. Dr. Rizvi will work in my laboratory, acquiring technical expertise in a wide variety of experimental approaches including use of genetically engineered mouse models of CCA, and patient derived xenografts of CCA (PDX). She will also become adept at cell biology approaches including confocal microscopy coupled with computer-based 3-D image reconstruction, expression and…

Inflammation is a defense mechanism where the body’s white blood cells fight off bacteria, viruses, and damaged cells(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . When pathogenic cells enter the body, the white blood cells surround that invasion site immediately(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . The white blood cells then release a specific toxic chemical that denatures the pathogen enough to kill it(Feeling the Heat- the Link between…

This can be seen when the flow Cytometry Department decide to send away a patient’s serum sample for an Anti-Neutrophil Antibodies Assay. When the serum sample is safely delivered, a confirmation fax is sent from the external source as seen in attached document 1. This form of communication…

Homework 4 1. A. Hcd19Tg is Human CD-19-Transgenic mice overexpressing CD19 which is a B-lymphocyte antigen present on all B cells. It is a transgenic model of human gene CD-19 introduced into the mice for research studying the effect of CD19 related to B-cell related immunological response B. CD1dloCD5+ is a rare phenotype of Regulatory B cells found in spleen of normal and autoimmune mice producing IL-10 and hence also called the B10 cells. As this phenotype is identifies to regulate T cell…

Why are you interested in working in this/these field(s)? Health care access and treatment of patients are incredibly important in everyday life, as well as tied to so many aspects of social, political, and economic discussion, which is one thing that has captured my interest. The opportunity to see how health care is run in a different country, as well as to see first-hand the daily life of a doctor, would be incredibly impactful for my future interests. Through school, I have gained knowledge…

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…