Flow cytometry

Specific Aim1: Investigation of in situ NK cell and neutrophil responses during MmuPV1 infection and tumor development Rationale: Innate immune cells including NK cells, monocytes, neutrophils, macrophages, and DCs play a crucial role during the early phase of the viral infection (36, 40, 62). We hypothesize that differential disease outcomes are associated with local immune cell populations and their activity. Neutrophils are the first and most abundant cell population that reaches sites of…

the increase of the MOI of the Pichinde virus. Fig 1.0(C) shows the results of a flow cytometry for the changes in the glycosylated α-DG for the LCMV and Pichinde virus at the different MOIs. These results are in accordance with those from Fig 1.0(B). Lastly, to test the down regulation in α-DG, HEK293T cells and human lung epithelial (A549) cells were infected with either LCMV cl-13 or Pichinde. Those flow cytometry results showed that there was a decrease in the glycosylated α-DG in both…

returned to the oncology clinic today to follow up on her lymphadenopathy, and several studies that were performed recently. One was a bone marrow (aspirate and) biopsy, and this was negative for any malignancy. There was (a lymphocytosis), but flow cytometry did not identify any malignant forms. Fortunately, no evidence of leukemia or lymphoma were identified. At the time the bone marrow was done, her platelet count had dropped significantly to 27,000. It had been over 300,000. The bone…

using this intentionally to its benefit and which pathways were utilized. The methods used included viral infection of cell cultures, hemagglutination assay, real-time quantitative PCR, plaque assay, transmission electron microscopy, TUNEL assay, flow cytometric analysis of apoptosis, western blot, subcellular fractionation extraction, inhibition of apoptosis using specific inhibitors, and cytokine and chemokine quantification. The replication efficiency of H1N1, H5N1, and H7N2 in P815 mast…

A/chicken/California/431/00 (H6N2), A/duck/Memphis/546/1974 (H11N9) and A/Puerto Rico/8/1934 (H1N1) at different time points and the percentage of infected cells will be determined using the Operetta High Content Imaging System (Perkin Elmer) or by flow cytometry…

know how many of the t cells will be using v beta 17. those put those t cells in a test tube in suspension and add some antibody tagged with green dye. The question then is how many T cells have bound the antibody. They used a technique called flow cytometry that takes a minute or two to determine how many T cells glow green. It takes that suspension of cells and it creates a stream of cells where all the cells are lined up flowing through a chamber. These cells travel at a very high rate at…

significant change to the platelet count that has been established to be 10 µM. Such was confirmed by the flow cytometry that exhibited the highest increment in larger platelets, with increase ranging between 10 and 20 µM of ADP. However, 20 µM could not be used because it would cause excessive aggregation. Platelets contained in population 1 (P1) area are all of the same size as that of the resting platelets and they are assumed not to be activated. Whereas, the platelets in population 2 (P2)…

The influenza virus was infecting its hosts long before the discovery of viruses in the late 1800’s. The longevity of the virus can be attributed to its ability to quickly mutate its genome, which consists of only eight negative-sense RNA segments. These small mutations allow the virus to rapidly evolve. In fact, approximately every twenty to thirty years the virus evolves into a “super virus” capable of causing significant pandemics (Webster, 2014). The influenza A virus is the envelope virus…

Paroxysmal Nocturnal Hemoglobinuria (PNH) is the rare thrombophilic and hematopoietic stem cell disorder with an annual incidence rate of as low as 1-2 cases per million. About 16% of these patients present with visceral vein thrombosis which is the most common cause of mortality. We here present an extremely rare case of a young man presenting with extensive thrombosis of multiple visceral veins from PNH. A 36-year-old Hispanic man with no significant medical history presented to the emergency…

BIOLOGY There are a few ways in which the process of sex selection can be completed. Though the most efficient way to use the Microsort flow cytometry method. A fluorescent dye is used to stain the sperm in a way that allows DNA bearing X and Y-chromosomes to be seen more clearly whilst a laser beam is passed through the stained sperm cell. This strategy has been utilized as a part of numerous configurations for more than 30 years. These strategies deal with the reason that they isolate the X…