Fast protein liquid chromatography

How Ferritin was first isolated and how it is isolated now The protein ferritin is formed from two classes of subunits, H and L, in a ratio which vary in different cell types. The H-type are subunits associated with rapid uptake of iron and are predominantly from the red blood cells. Subunits that take up iron much slower are designated L-types. A third type subunit, M, is found in amphibians and is similar to the H subunit. The M-types are predominant in the liver. Once synthesized, ferritin…

used to determine methamphetamine and its metabolites. Since amphetamines are weak bases, their ionic form may bind to the sorbent. However, the mixture of organic solvent and alkaline solution, which is utilised for elution, must be removed before liquid chromatographic analysis. (Lee et al. 1997; Wood et al. 2005; de Castro et al. 2013) Moreover, the eluted solution may contain many compounds that interfere with the analysis. Therefore, cation exchange cartridges are replaced by mixed-mode…

Vaccination is the process of injecting antigenic material to stimulate immune response. Conventional vaccines insert attenuated or killed infectious agent, such as virus or antigenic protein, into the body to stimulate immune system to develop immunity against the pathogen. However, this type of vaccines has some weakness. For example, attenuated virus may suddenly become very active in the body and cause disease instead of stimulating immune response. Conventional vaccines also unable to…

The purpose of this research is to understand how secondary structures of a protein, in this case sperm whale apomyoglobin, affect the denatured state of that protein. The scientific community has been working under the impression that once denatured by urea, a protein loses all of its secondary structure. In this research the effect that apomyoglobin’s history of secondary structure has on the denatured state of the protein will be determined through kinetics of the refolding reaction. The…

Medicine and seven different fast food places. This lawsuit regarding the Preposition 65 which states that if there is anything in a food product the restaurants are obligated to inform everyone regarding it with a clear warning. Another observation that sparked her research was while she was eating grilled chicken marinated in lemon juice. She noticed that the edges were white which she found out that it was called denaturing. Denaturing is a process in which the proteins will change shape and…

Pollution is one of the most serious problems that affect our environment, and its effects on all life aspects are clear to everyone. Because of this, pollution is one of the most serious issues that all the word countries are concerned about. As time goes, the pollution problem increases and also the population growth increase. The increase in demand for water and food supplies place an increasing stress on the ground and surface water quality and quantity. One of the environmental problems…

DNA fragments located inversely oriented. DNA segments of about 100-3000 bp amplified by PCR and flanked by closely spaced microsatellite sequences (DNA repeated sequence are short in specific locus) by a single primer or a pair of primers. ISSR is fast, effective and highly informational technique. They are considered reliable for DNA fingerprinting and by using Random Amplified Polymorphic DNA (RAPD) technique can be recognized to detect any genetic changes at the individual level as well as…

SDS-PAGE gel was used to analyze the protein samples from the MBP-AP and WT-AP experiments. The samples are then referenced to the ladder to determine the molecular weight of the MBP-AP and WT-AP proteins. Then the UV absorbance of the two proteins from 240 nm to 340 nm is determined using a nanovolume cuvette. The absorbance at 280 nm was then used in conjunction with data from previous experiments to determine the concentration of the MBP-AP and WT-AP protein samples. Results of experiments…

include anabolic androgenic steroids, testosterone, human growth hormone, erythropoietin and other androgens (Grace & Baker, 2012). These drugs for example anabolic androgenic steroids imitate the activity of natural testosterone and lead to fast rate protein production by muscle cells and thus achieving bigger muscles and increased endurance. The illegal use of these products to gain a competitive edge is doping. According to my research, this practice has been on the rise for decades now not…