Fast protein liquid chromatography

Introduction: Centrifugation is a process used to separate or concentrate materials suspended in a liquid medium. It is a method used to separate molecules under the centrifugation field based on their sedimentation rate with the help of centrifugal force. It is also used to measure the physical properties of a molecule like molecular weight, density, shape, etc. If it is used for the separation of one type of materials from others is termed as preparative centrifugation and when used for…

Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…

High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and…

Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…

CHAPTER-V FORMULATION AND EVALUATION OF LEVODOPA FLOATING TABLETS FORMULATION AND EVALUATION OF LEVODOPA FLOATING TABLETS Several approaches are currently used to retain the dosage form in the stomach. The principle of the floating tablets is simple and practically approach to achieve increased gastric residence time to obtain controlled release floating tablets designed based on the gas generating principle needs a strong floating matrix former. In this regard HPMC K4M,…

The invention relates to formulation of Ketorolac Tromethamine and Phenylephrine Hydrochloride immediate release tablet having less disintegration time. HPLC method was developed and validated for determination of content of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. UV spectrophotometric method was developed and validated to study drug release profile of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. Background:…

kept in a boiling water bath for 10 minutes. All the tubes were then cooled under running tap water and finally readings were taken at 570 nm against reagent blank. The catalase activity in gastric tissue was expressed as µmoles H2O2 consumed /mg protein /min. 4.2.3.5.2. Glutathione peroxidase activity Estimation of glutathione peroxidase was made according to the methodology of Flohe and Gunzler, 1984. Took 0.2 ml of EDTA, sodium azide, reduced glutathione and H2O2 in a test tube. Then added…

Protein Separation Using Gel Filtration Chromatography Introduction: Chromatography techniques are one of the most useful methods use for the isolation or separation of macromolecule from a mixture. The most common used chromatography techniques are thin -layer chromatography (TLC), column chromatography, etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture based on their size. Also, collecting elutes that run through…

Aim: Discuss the advantages, disadvantages and limitations of mass spectrometry for the analysis of both small molecules and proteins. Background Mass spectrometers. They are a powerful tool used to evaluate analytes such as single atoms, molecules, whole proteins, or the simple peptide chains that make them up. Using the mass to charge ratio (m/z) of these analytes, we can differentiate one or many entities (such as one particular peptide fragment, or a panel of them) from everything else in…