Ethyl acetate

with alloxan induced diabetic rats and normal rats. The result of antidiabetic potential suggested that methanolic extract possess antidiabetic activity. Methanolic extract processed through bioactive fractionation as water soluble fraction and ethyl acetate fraction. The phytochemical investigation and antidiabetic activities was carried out on…

has a low ß-lactam binding affinity. Therefore, it can mediate cell wall synthesis even in the presence of ß-lactam drugs (5). mecA Homolog mecC gene Recently, mec C gene was found to confer ß-lactam resistance in SCCmecXI-carrying S. aureus. The mec C gene was found to share 70% similarity to mec A. Where as mec C encoded PBP2A homolog PBP2C, shares only 63% identity with PBP2A protein. In order to prove its role in Methicillin resistance, a knockout strain was generated.These stains have…

groups did not gain an additional proton from the previous reaction. Next, the solution was filtered in a Hirsh funnel. Then, acetic anhydride was added to the filtered solution, causing the solution to turn a light orange-pink color. Finally, Sodium Acetate was added to the solution causing the solution to turn a white cloudy color. The final result of this reaction was crude Phenacetin from…

Introduction Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…

wheat kernels in 150 μm sieve; Wwheat in 75μm sieve = weight of wheat kernels in 75 189 μm sieve; Wpan = 190 weight of empty pan; and Wmaize meal = weight of maize flour. 191 Determination of Crude Protein 192 The crude protein content of the roasted maize was determined in duplicate by Dumas combustion 193 method as described in AACCI Approved Method 46–30.01 (AACC, 1999) using a Leco, model 194 FP-528 (Leco Africa, Kempton Park, South Africa). Calibration of the instrument was done using 195…

obtained from Toronto Research Chemicals (North York, Canada). Sulfuric acid 95–97% (both Baker-analyzed grade), isopropanol (Baker HPLC analyzed) and methanol (HPLC gradient grade) were purchased from Mallinckrodt Baker (Griesheim, Germany). Ethyl acetate and NaHCO3 were supplied by Merck KGaA (Darmstadt,…

The purpose of this lab was to use NMR, Recrystallization, TLC, and Melting point to separate and identify the components of a given unknown mixture. The first step of the experiment was to use the two NMR spectra given to identify the major and minor components of the unknown mixture. When an external magnetic field is applied, there is a quantized interaction between the magnetic field itself and the magnetic moment of the proton. Thus, the magnetic moment of the proton either applies with or…

Table: 9 FT- IR analysis of methanol extract of M. concanensis leaves S. No Peak values Intensity range Intensity Group assignment Functional groups 1 3972.43 0.0086 Unknown 2 3859.33 0.0412 Unknown 3 3805.79 0.0733 Unknown 4 3396.46 0.9280 Medium N-H stretch Amides, alcohols and amines 5 2987.43 0.2450 Medium C-H stretch Alkanes and acids 6 2946.27 0.2810 Medium C-H stretch Alkanes and acids 7 2912.38 0.2040 Medium C-H stretch Alkanes 8 2834.49 0.3170 Weak C-H stretch Aldehydes and…

high according to their observed and theoretical melting points. Both substances melted over ranges matching their theoretical melting points, most likely indicating an absence of impurities. Recrystallization of the acetylsalicylic acid in ethyl acetate likely contributed to the recovery of a purer sample of acetylsalicylic acid. The recovered acetylsalicylic acid, with a theoretical melting point of 138° C - 140° C, melted between 139° C and 146° C. Deviations from the theoretical melting…

The motivation behind this experiment was to gain insight behind the theory and practice of two laboratory techniques: solubility-dependent extraction, and recrystallization, in an effort to isolate and identify three compounds originally dissolved in a mixture. Extraction is used to isolate a specific compound. Recrystallization involves taking a crude compound and removing unwanted impurities so that a more accurate melting point can be taken, leading to a more precise identification of a…