6. EXPERIMENAL WORK 6.1 Preliminary Work 6.1.1 Collection of Plant material The leaves of Jasminum grandiflorum was collected locally in the month of July locally from Bhopal, Madhya Pradesh, India. Herbarium file of plant part was prepared and authenticated by Dr. Zia Ul Hasan (Professor, Department of Botany), Safia College Bhopal and the specimen voucher no.assigned was 344/Bot/safia/15 6.1.2 Drying and Size Reduction of Plant Material The leaves of Jasminum grandiflorum was dried under…
CHAPTER 4 RESULTS AND DISCUSSION 4.1 Extraction using Different Solvents The grounded Ixora coccinea and Beta vulgaris plant samples were extracted using the following solvents: Acetone, ethanol, methanol and ethyl acetate. The absorbance was noted to select the best choice of solvent for extraction. Inference Acetone, ethanol and methanol showed higher values. But, the dye extracted from acetone and ethanol did not fix to the fabric properly. So, methanol was selected as solvent for…
Jatropha gossypiifolia L. (Euphorbiaceae), widely known as “bellyache bush,” is a medicinal plant largely used throughout Africa and America for treatment of inflammatory analgesic antidiarrheal diseases. In spite of some published data on its efficiency, there are still few toxicological data unfolding the safety of this plant. The plant was extracted with many solvents (pet. ether, ethanol, and water). The preliminary phytochemical outcomes revealed that flavonoids and other glycosides as…
3.3.3: Phytochemical analysis The leaves and bark extracts were tested for some secondary metabolites like-saponins, tannins, alkaloids, anthraquinones, phlobatannins, flavonoids, terpenoids, reducing sugar and poly phenols. Test for reducing sugar: Mixture of 1ml or 1gm of plant sample is taken in a test tube and 10ml deionized water is added in it after this a few drops Fehling solution (1ml Fehling solution A and B) is also mixed and heated at 1000C in a water bath. The Brick red…
Gold electrodes were prepared after cleaning with H2SO4 and H2O2. Glucose oxidase and laccase were immobilized on the GO/Co/chitosan modified electrodes in a sodium phosphate buffer solution (0.1 M, Ph 7) for 8 h [12]. The electrochemical activities of enzymatic electrodes were measured using VersaSTAT3 (AMETEK, Princeton Applied Research, USA). Electrochemical measurements such as CV and EIS were carried out using an Au electrode with the assembled glucose oxidase, Ag/AgCl, and a Pt wire as the…
Purpose In this experiment, volumetric glassware was used in order to determine the density of an unknown liquid. Additionally, the opposite process was used to determine the calibration of a piece of glassware using a known density. In the first activity, “Density of Liquids”, the identity of a liquid was determined by calculating its density (g/mL). First the mass (g) of a specific volume (mL) of water and the unknown liquid was found. Their densities (g/mL) were then calculated by dividing…
Introduction: As it is taught throughout school, all living things are made up of cells. These cells are carry out all the necessary functions to keep the human body well maintained including chemical reactions and other complex tasks. In order to carry out these functions, it’s vital for the cell to possess a membrane. Cells create cholesterol, which is a cyclic fatty-product that maintains the cells permeability. Cholesterol is also synthesized to create steroid hormones, and also vitamin D.…
7. RESULT & OBSERVATION Table No.7.1:- Morphological characteristics of Jasminum grandiflorum leaves S.No. Character Observation 1 Color Gggggggg Green 2 Odour Characteristic 3 Taste Acrid 4 Size 4-5.5 cm. length 5 Texture Smooth Table No.7.2:- Physiochemical analysis of powder of Jasminum grandiflorum leaves S.No. Parameters Observation (%) 1 Loss on drying 0.6 2 Total ash value 7 3 Acid insoluble ash value 2.6 4 Water soluble ash value 1.25 5 Foaming index 22 (ml)…
The purpose of this experiment was to determine the presence of the analgesic compounds acetaminophen, aspirin, caffeine, and salicylamide in the over-the-counter medications Anacin, Bufferin, Excedrin, and Tylenol. Thin-layer chromatography (TLC) was used to analyze solutions of the medications; the TLC plates were visualized using ultraviolet light and iodine staining.The ten micropipets used to spot the TLC plates were made from glass capillary tubes by heating the middle of each tube in a…
known as, Diamorphine Hydrochloride Anhydrous, heroin is a hydrochloride salt of a diacetyl derivative of morphine. Because of the chemical structure of heroin, it is usually found in the form of white crystals or crystalline powder from prisms of ethyl acetate. After administration, the heroin is “rapidly hydrolyzed to 6-monoacetylmorphine and then to the end-product morphine” (Diamorphine). This morphine then binds to the opiate receptors in the brain and digestive system. Since heroin is…