Essential amino acid

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    Proteins that play an important role in the amino acid metabolism are seen in f.alocis . although it lacks some inherent amino acid synthesis pathways it could alternate through protein degradation with the help of these proteases and peptidases. Also f.alocis has proteins responsible for ornithine catabolism and urea breakdown. So we can say that f.alocis has a wee developed nitrogen assimilatory pathway that is needed for alternative mode of amino acid synthesis.so we can conclude that…

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    Yeast Expression

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    improves the affinity tagging of the proteins, reduces protein breakdown, allows the addition of non-native amino acids (Selenomethionine) into the protein and defines the composition of N-glycan in the protein produced. Following are the existing genera of yeast…

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    Cellular Respiration takes place on the level of the cell, inside the mitochondria. This is the process of breaking down food in the presence of oxygen to form ATP. ATP stands for Adenosine Triphosphate which is an energy bearing molecule found in all living cells1. Things such as bacteria can perform this with their outer membranes. When a person runs, they use cellular respiration to create energy in the form of ATP which allows their muscles to move. Many other energy-consuming reactions of…

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    Csrn1 Case Study

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    In this study, we analyzed the bulk of genomic and transcribed CsRn1 sequences encompassing the putative frameshift region in C. sinensis and Opisthorchis viverrini, which is a nearest phylogenetic neighbor of the liver fluke, to address the issue regarding the expression strategy of CsRn1. Our results demonstrate that CsRn1 copies lacking frameshifting have arisen from the precedential counterparts with two overlapping ORFs by a single-nucleotide insertion at an upstream region of gag stop…

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    Introduction Drosophila melanogaster can be considered model organisms for the study of genetics. Some characteristics that contribute to this status are their quick lifecycles, large amount of offspring, and the presence of only four chromosomes. Drosophila melanogaster contain bristles on the thorax, head, legs, and abdomen. These bristles are used as a sensory mechanism, allowing fruit flies to detect their surroundings as they fly and walk around their environment (Yehuda, 2011). The rate…

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    for both microassays and standard solutions. The analysis provides a linear response with concentrations of Bovine Serum Albumin (BSA) from 1 to 140 microliters. The reagent used was the combination of Bradford Reagent-Brilliant Blue G in phosphoric acid and methanol. The dye molecule is mostly deprotonated and converted on the process to a reddish colored species that have the potential to bind to other proteins. Once this red colored species bind to a protein the bound dye molecule transforms…

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    OMEGA-3 FATTY ACIDS 1) Structure Figure 1.1 The omega-3 fatty acids are also known as ω-3 fatty acids or n-3 fatty acids which contain a long chain of polyunsaturated fatty acids and the chain ranges from 18 to 22 carbon atoms (Collins J.J., 2010). The fatty acids have two ends; the carboxylic acid end (-COOH) and the methyl end (-CH3). The carboxylic acid end is considered the starting of chain which is “alpha” and methyl chain is considered as the tail of the long chain…

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    Leroy et al. (2000) used 4 microsatellite primers to characterise Brassica oleracea accessions. Among the 136 reproducible fragments generated, 25 (18.4%) fragments were common for all Brassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Flannery et al. (2006) assessed polymorphisms in Brassica, Arabidopsis, Camelina, Raphanus and Sinapis using 10 plastid SSR primer sets. Eight loci were polymorphic, and separated the individuals of Brassicaceae into taxon-specific…

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    Aim 2: How does folliculin detect amino acids to regulate mTORC1 activity? Here, the goals are to elucidate the mechanism of action that enables folliculin to function as an amino acid sensor. 2.1. Determine the amino acid reservoir folliculin senses. Evidence points FLCN as a novel amino acid sensor. To resolve the biochemical mechanism FLCN employs, we need identify the specific amino acid reservoir FLCN senses. Lysosomes’ are the main amino acid reservoir and the ‘inside-out’ mechanism is a…

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    Introduction So what is GGT? GGT stands for Gamma-glutamyl Transferase, it is found on the cell surface that contribute to the extracellular catabolism of glutathione (GSH). The most GGT serum is derived from the liver, but it is also produced in many other tissues. GGT is primarily carried with lipoproteins and albumin in serum. The levels of GGT is determined by various factors such as alcohol intake, body fat content, plasma lipid/ lipoproteins and glucose levels, and also various medications…

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