Escherichia coli

when put under conditions similar to fermentation. LysA2, when tested on strains of bacteria that were not lactic acid bacteria, did not demonstrate any lytic activity. Along the same lines, no lysins had successful results when tested against Escherichia coli DH5alpha and Saccharomyces cervisiae Y-2034. The authors used pH and ethanol concentration as variables because within a real fermentation environment, all these factors are slightly variable. That necessitates testing the effectiveness of…

Elizabeth Casey Lab Partners: Cara Hull, Erin Magennis, Claire Pfeffer Bio2300 Section 1 10/6/16 Dr. Henle Testing Effects of Glucose and Sodium Chloride on E.coli Cell Division Hypothesis: We hypothesized that if E.coli cells are grown in broth containing 1% 1M glucose, then they will divide more rapidly than they would without the glucose additive. Our independent variable was the glucose, and our dependent variable was E.coli cell density. Methods: This procedure was obtained from the…

enzyme’s activity to the substrate. Noncompetitive inhibitors bind to other sites on the enzyme thereby changing its shape for blocking the substrate (OpenStax Biology, 2017). In this lab, the enzyme beta-galactosidase was used. In the bacteria Escherichia coli, it is used to catalyze the breakdown of lactose into galactose and glucose and to convert lactose into allolactose. The enzyme can also be used in colorimetric assays with substrates such as ONPG, which is used in this lab. The product…

The scientific context of the the API 20E test is clinically used in quickly identifying enterobacteriaceae bacteria and gram-negative rods. In the Antimicrobial Susceptibility experiment, three microorganisms which were Staphylococcus aureus, Escherichia coli, and the unknown were put into three different agar plate and each plate were divided into four quadrants. In each of the four quadrant of the agar plates the antibiotics of ciprofloxacin, penicillin, Trimethoprim with sulfamethoxazole,…

onset after an infection of Escherichia coli which is a Shiga toxin-producing bacteria. There are two types of Shiga toxins that can lead to HUS, Shiga Toxin 1 (Stx1) and Shiga Toxin 2 (Stx2). There have been structural differences found between the two types as well as evidence to show that type 2 Stx causes more harmful kidney…

BIOL 1010 ASSIGNMENT 1 OCT 6 BY JORDAN KAPITANY ST 100883963 Among the many scientific achievements of the twentieth century in the field of bio-technology scientists Paul Berg, Herbert W Boyer, Stanley N Cohen and team for their research that lead the party to discover a technique of taking genes from one organism and inserting them into another organism, also more formally known as Deoxyribonucleic Acid or DNA recombinant technology. In 1971 Berg and team successfully isolated DNA of…

NECROTIZING FASCIITIS This is an uncommon disease. Necrotizing Fasciitis is also known as a flesh-eating bacteria disease. This affects every 1 out of 4 people. Some symptoms of this disease are weak immune system, consumption of steroid medicines, has surgical cuts or injuries on parts of their body, or had been affected by chicken pox recently. This bacteria destroys and eats up fat, skin and the tissue covering the muscle of a particular part of the body, and can occur anywhere in the body.…

Chalcone scaffolds had attracted many researchers to investigate their synthetic routes, potential biological activities and their roles as intermediates for naturally occurring flavanoid skeletons. This study focused on the synthesis of chalcones bearing O-, C-prenyl and chromane as substituents. The target compounds were purified by chromatographic methods and structures of all compounds were determined by using IR, NMR and MS spectroscopies. The prenylated precursors were synthesized…

One disease in this category is food safety threats like Escherichia coli (E. coli). We all know about this gram-negative bacterium mostly found in food, intestines, or environment. Popular food chain Chipotle had an outbreak in 2015 and resulting in disease surveillance for the next few years. Other diseases include Q…

In this extension experiment there were a few errors that occurred in some of the trials. This is to be expected as perfect sterile techniques are unable to be achieved in the conditions provided. This is also why it was chosen to do three trials of each antiseptic so when errors do occur they can be easily recognised as anomalies. In the Domestos trials the main error was in the second plate where no results could be found for any of the four sections. As can be seen in the photo a small…