Escherichia coli

HepG2 cells were obtained from American type culture collection (ATCC), USA (ATCC No. HB-8065). This cell line is derived from a liver hepatocellular carcinoma of a 15 year old Caucasian male. After thawing, cells were maintained as a sub confluent monolayer in a 75 cm2 culture flask using the modified DMEM medium with 10% (v/v) FBS + penicillin-streptomycin (50 units/ml). The cells were used to experiments after two weeks of thawing from cryopreserved stock. On the day of harvest, the cell…

Streptomyces and Antibiosis From our group, Group 2, nutrient agar plates inoculated with E. Coli, (Images 1.1,1.2), (Images 2.1,2.2), (Images 3.1,3.2) no antibiotic activity was detected. This could be a result of the putative colonies chosen, not being Streptomyces. All selected colonies were white, or pigmented rink, raised and rough. One (1) and three (3) were inoculated with the putative Streptomyces colonies. Plate 2 was inoculated with the control Streptomyces colony. Although it was…

1. Where do restriction enzymes come from and what is their original purpose? Restriction enzymes come from bacteria. Bacteria used restriction enzymes to cut up DNA into fragments to fight off viruses. 2. a. Why do you get different fragment sizes if you cut the DNA from two different species with the same restriction enzyme? Each species has a different arrangement of bases, and the restriction enzyme only cuts after a specific order of bases. Due to that being the case, the specific…

Abstract The purpose of this experiment was to use 3 different techniques (Direct count with hemacytometer, viable count, plate count, turbidity study using spectrophotometer) to calculate the cell count and growth of bacteria, while using serial dilution. The 3 techniques were used to calculate the % viability of Serratia marcesens in a solution. Introduction Enumeration of microorganism is used to determine the amount of bacteria present in a sample. Microbiologists around the world are…

occurring outbreak of E. coli O157: H7. Bob Nugent President…

Potter (2001) mentioned that C. perfringens spores may contaminate meat and meat products either before processing and survive cooking or after processing due to unhygienic handling of prepared food. Adak et al. (2002) reported that deaths due to C. perfringens (type A) food poisoning are rare but may occur in the elderly and debilitated. It is estimated to kill seven people in the USA, and between 50 and 100 people in UK yearly. Asha and Wilcox (2002) reported that the first C. perfringens…

Antibiotic is a medicine that inhibits the growth of or destroys microorganisms. April 20th our class was assigned to do experiment. First step, Dr.Okeke went through the lab rule with our class. Second, our class had to get in groups of 6. Third step, Dr.Okeke gave everyone safety goggles, gloves, and mouth mask. Safety goggles are forms of protective eyewear that usually enclose or protect the area surrounding the eye in order to prevent particulates, water or chemicals from striking the eyes.…

Foodborne illnesses cost the United States billions of dollars each year. There are five major bacterial pathogens that cost the United States the most, which are: campylobacter. E-coli 0157:H7, E-coli non-0157:H7 STEC, Salmonella, and Listeria monocytogenes.According to fightbac.org, salmonella is the most costly, coming to a total of around 2.65 billion dollars each year in the United States. Campylobacter costs the US around 1.2 billion each year as well. People and/or the government spend…

Cysticercosis Cysticercosis is an infection caused by the young form (larvae) of a tapeworm (Taenia solium) that can be found in pork. When eggs from this tapeworm are consumed, the eggs develop into larvae inside of the body. Larvae can spread through the body to areas such as the eyes, skin, muscle tissue, heart, and brain. When cysticercosis occurs the brain, the condition is called neurocysticercosis. Severe infections in the brain can be life-threatening. CAUSES This condition is…

The morning of November 9th, 2016, citizens of the United States awoke to learn that Donald Trump would be the next President of the United States. Some cried, some cheered, as for me, I felt numb as I sat looking at my phone in disbelief. As I reflected on the past few months, many thoughts rattled around my head and disappeared, but one message remained. Donald Trump didn’t win the election because he knows politics and how to lead the most powerful nation on planet earth, he won the election…