Escherichia coli O157:H7

In everyday life, people use disinfectants, substances used to destroy viruses and microbes such as bacteria and fungi1, to clean their bathrooms. If not properly sterilized and cleaned, the bathroom can be a domain for numerous bacteria including ubiquitous Serratia Marcescens, a short, rod shaped, facultative anaerobic, opportunistic pathogen that can cause nosocomial infections such as urinary tract infections, bacteraemia, meningitis, sepsis and cerebral abscesses2. Facultative anaerobic…

particular lab, students will be taking the pGLO gene, which makes the protein Green Fluorescent Protein (GFP), commonly found in a species of jellyfish, which glow (N.d., p. 32). Theoretically, when the pGLO gene is inserted into the E. coli bacteria, then the E. coli bacteria will also…

Sulfur not reduced. 5a. Lactose fermented. 6a. Sucrose fermented. 7a. Ornithine decarboxylase present……Enterobacter aerogenes 7b. Ornithine decarboxylase not present…….Klebsiella pneumonia 6b. Sucrose not fermented……………………………….Escherichia coli 5b. Lactose not fermented…………………………………….Serratia marcescens 1b. Glucose not fermented 8a. Glucose oxidized…………………………….........…………Pseudomonas aeruginosa 8b. Glucose not oxidized…………………………………………......Alcaligenes faecalis Test Question…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

showed positive results indicated by the red color of the broth and the VP test showed negative results which were indicated by the lack of red color when adding the dye. The results of the MR/VP test lead me to the conclusion that my unknown was Escherichia coli but to be…

The role of this structure in the pathogenesis of the bacteria is of high medical importance as it helps the bacteria to be propelled or display from one location to another carrying the infection to other areas fast, an example is the Escheria Coli, which can propel from the urethra to the bladder, known as the most common causes of urinary tract infection (UTI’s), and some other as flagellum cannot be seen under microscope without stain, helps to differentiate them using specific antibodies…

Due to limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral…

These results lead to the identification of unknown 18X as Escherichia Coli. The Gram test was first performed on the provided culture of unknown 18X. To double-check the results, a second Gram test was performed from the incubated nutrient broth that was grown from a colony of the streak plate. It provided the same…

A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…

the patient, unknown bacteria #618 was identified to be Escherichia coli. It is apart of the Enterobacteriae genus that according to Brown’s Microbiological Applications1 are gram-negative bacteria that usually live inside the intestines of animals and humans. The organism can also live in the vagina and urethra. The data collected after isolating the organism and executing a series of tests have confirmed previous observations of E. coli in past scientific history. For the majority of the…