Escherichia coli O157:H7

coli with pGLO plasmid that codes for Green Fluorescent Protein (GFP). The pGLO plasmid encodes a fluorescent protein that glows green when exposed to ultraviolet light. Following transformation, the bacteria express their newly acquired gene and produce…

1000875555 Mofetoluwa Adeosun What effect does Packaging have on the quality and safety of Sardines? Fish is a highly perishable food whose shelf life is limited to the concentration of atmospheric oxygen and the growth of spoilage microorganisms. A technique involving the modification of the atmosphere within the package reduces the oxygen concentration while increasing the carbon dioxide concentration, leading to an increase in the shelf life of the highly perishable food at chilled…

Salmonella is very common, more than 1,000,000 people are infected annually(CNN). Salmonella is most common in South America due to a lot of meat and poultry consumed there. The salmonella bacteria affects the Digestive System, it also affects the vitals if it reaches the bloodstream. The digestive System is in charge of breaking down the food that is consumed, and if the Salmonella bacteria is in it it won’t be able to do its job. The Blood stream carries blood around the body, if salmonella…

Amoxicillin is an beta lactam aminopenicillin that inhibits bacterial cell wall synthesis by binding to penicillin binding protein and blocking transpeptidation. It has low protein binding and is eliminated renally and mostly unchanged. It has a beta lactam ring, an absolute configuration of 2S, 5R, 6R, a carboxylic acid group at C2 position and an amide bond at the C6 position. It has broad spectrum activity and can be used against gram negative bacterial infections. Erythromycin is a…

5.1 Conclusion The bathroom stands as an important point in the transmission of infection in the community and the entire environment as it houses various varieties of microorganisms, which may be potential pathogens or pathogenic themselves, in the form of biofilms, and may become a threat to immono-compromised or ill persons as shown by the results of this study. Continous use of these bathrooms without knowing the different type of organisms colonizing these areas therefore, is a public…

Bacteria are microscopic single-celled organisms that thrive in diverse environments. In the American Journal of Gastroenterology, the authors mention that gut bacteria and other microorganisms aid in digestion and help in the development of the immune system. Other bacteria can cause infections (Vidyasagar Aparna, 2015). Infection is caused by pathogens such as bacteria, viruses, protozoa or fungi getting into or onto the body (Department of Health & Human Services, State Government of Victoria…

After my gram staining I was able to comfortably eliminate all of the gram negative results. This encouraged me in knowing that I was able to execute an experiment efficiently enough to eliminate some of my choices. I was also able to eliminate all of the streptococcus bacteria because my bacteria was in clusters and not in a chain arrangement. Gram staining also helped me in eliminating the bacilli because my interpretation of the observation was that my unknown bacteria was spherical in nature…

Many microorganisms are beneficial, for example, some in our large intestines synthesize vitamins and allow them to be absorbed into the bloodstream. A tiny minority are pathogens (disease-causing agents). Pathogens are also known as germs or bugs and can be life threatening. External injuries is the bacteria’s entrance to the body. They get energy from molecules such as sugars. Bacteria uses ATP for growth, movement, temperature regulation, and many other processes. They grow best in unhygienic…

HepG2 cells were obtained from American type culture collection (ATCC), USA (ATCC No. HB-8065). This cell line is derived from a liver hepatocellular carcinoma of a 15 year old Caucasian male. After thawing, cells were maintained as a sub confluent monolayer in a 75 cm2 culture flask using the modified DMEM medium with 10% (v/v) FBS + penicillin-streptomycin (50 units/ml). The cells were used to experiments after two weeks of thawing from cryopreserved stock. On the day of harvest, the cell…

Streptomyces and Antibiosis From our group, Group 2, nutrient agar plates inoculated with E. Coli, (Images 1.1,1.2), (Images 2.1,2.2), (Images 3.1,3.2) no antibiotic activity was detected. This could be a result of the putative colonies chosen, not being Streptomyces. All selected colonies were white, or pigmented rink, raised and rough. One (1) and three (3) were inoculated with the putative Streptomyces colonies. Plate 2 was inoculated with the control Streptomyces colony. Although it was…