Escherichia coli O157:H7

1. Problem to be addressed. To study the effect of a paraprobiotic, heat inactivated Lactobacillus gasserei, on the functioning of the central nervous system using mouse models. 2. Background to the problem. The microbial composition of the gut in early life is known to influence the development and functioning of the adult brain, including behavioral functions. Many behavioural disorders like anxiety, depression and autism have been linked to severe damage to the gut microbiota. Studies have…

“An ounce of prevention is worth a pound of cure” (Benjamin Franklin). Listeria is a serious foodborne illness. It is caused by a bacterium, called Listeria Monocytogenes, that lives in soil, water, and certain animals. It is especially prevalent in poultry and cattle. Listeria can also be found in unpasteurized milk, and products that are made with raw milk. Listeria is especially dangerous because it is able to grow, even in cold temperatures. Refrigeration will not kill the bacteria. Listeria…

The purpose of this experiment was to determine the working concentration of Tetracycline (TNC) on Escherichia coli (EC). We used EC because of its characteristics of fast growth in different environments, complete gene sequence, and availability. Our antibiotic, TNC, is a broad spectrum antibiotic which is effective to many types of bacteria. This is because it obstructs tRNA anticodon from reading the mRNA codons in the 30S ribosomal subunit which halts the lengthening of the protein chain.…

The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…

AIM: To create competent E.coli cells by using the chemical method (calcium chloride treatment) To transform the competent bacterial cells using plasmid DNA and evaluate the transformation efficiency. PRINCIPLE: Competent cells are pre-prepared bacterial cells that possess altered cell walls through which foreign DNA can enter the cell easily. Most cells cannot take up DNA efficiently unless they have been treated with chemicals or exposed to electrical treatments in order to make them…

Although bacteria are often seen as something that is bad for you, it can also be good. In fact, it is in your stomach; it’s practically everywhere. To test the ubiquity of bacteria, my class and I conducted an experiment. We sampled random surfaces like ears, mouths, cell phones, soil, shoe, desk, bathroom door handles, and as our constant variable we left one side of our TSA plate blank. This should tell us whether or not our hypothesis is correct. My hypothesis was, “bacteria is found in…

The gas generation is due to E. coli metabolic processes that generates acid and changing the turbidity. 2. The Membrane Filtration Method did work as well. MF method can replace the MNP test for microbiological analysis of water sample. After the incubation of the mEndo plate for 24 hours it is perceptible the grown of E. coli colonies across the plate. The results of this method are shown in the figure 4. As it was used mEndo, just the E. coli colonies form bright green colonies with…

ABSTRACT: The Microbiology & Immunology Laboratory Unknown Identification Project is designed to test the students’ skills and knowledge of performing biochemical tests learned during the course of the lab. Students receive a numbered tube containing a mixed culture of two unknown organisms and are required to determine the identity of each microorganism by performing a series of biochemical tests of their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain…

The Process of Ligation, Transformation, and Isolation of EGFP cDNA Into pET-41a(+) By Using Miniprep, Restriction Digest, PCR, and Bioinformatics To Ultimately Create Recombinant Expression Plasmids. INTRODUCTION: Scientists in this day and age are now able to change DNA and create recombinant DNA, which is a huge indication of great possibilities for the future of genetics. The goal of these lab experiments was to be able to create recombinant DNA with the insert of egfp gene. EGFP also…

Antibiotics are very useful and powerful drugs that help kill many different infections. In the 1940’s, before antibiotics were invented, many people died from bacterial infections (Goldsmith 7). “In the past twenty five-years, the widespread misuse of antibiotics has turned many formerly curable bacterial diseases into nearly untreatable ones” (Goldsmith 9). “Bacteria have shown the ability to become resistant to every antibiotic that has been developed” (CDC 41). According to the Centers…