Escherichia coli O121

Perianal Dermatitis Dermatitis is redness, soreness, and swelling (inflammation) of the skin. Dermatitis that occurs around the anal opening is called perianal dermatitis. This condition can be passed from one person to another (contagious), depending on the cause. CAUSES Perianal dermatitis may be caused by a type of germ (bacteria) infection. Streptococci bacteria are the most common cause of this infection. These bacteria can be found in the throat where they can cause strep throat. These…

the presence of Arabinose (Ara). The GFP is expressed after transformation such as a brilliant green color when it was seen in the UV light. The transformation efficiency describes how effective was the transformation of the pGLO added into the E. coli bacteria. The transformation efficiency should be a very high number of transformation efficiency. In this experiment, the transformation efficiency are the followings: LB/Amp (+DNA) was 20307.69 and the LB/Amp/Ara (+DNA) was 11538.46 of…

This was avoided by introducing the ampicillin strain into the E.coli cells which made them ampicillin resistant. Thus by transfection of the DNA, E. coli was able to take up the strain into its genome and recombination occurred as the cell became ampicillin resistant. That is why colonies were able to form on the agar plates. All the experimental plates had colonies including the agar plate of the enzyme…

1) Figure 1 shows the test results that was entered in to the Api system to give possible causes of a bacterial urinary infection. Three microorganisms where listed, Escherichia coli 1, Serratia odorifera and Klebsiella oxytoca but gave an unacceptable profile due to in all three cases failing to get negative result in the TDA test with detects the use of tryptophan deaminase activity and the NO₂ test, turning nitrates in to nitriles. E.coli is one of the most common causes of an urinary…

“Sometimes things are hard, and some things are right. And some things are both. This is one of those things.” –Tim Cook Now when Tim Cook said this he was in no way referring to genome editing, but his words can be applied to many difficult situations. Imagine a world where doctors are gods creating and designing the next generation of human beings. Before a baby is even born they are able to go in and pick and choose what the baby will look like, what abilities they have. They can remove…

Did you know that about 280,000 people die annually due to being overweight? Most people don’t know how eating unhealthy can cause major difficulties in their later years. Eric Schlosser is an investigative journalists, who wrote the nonfiction book called “Fast Food Nation”. The book is about the global and local influences the United States’ fast food industries have. Although some may argue that the corporations should led a reform of the US food system, overall, the government should take…

gives a variety of different results that are easy to interpret. A metallic green color indicates either Escherichia coli or Citrobacter freundii, purple can indicate Enterobacter aerogenes, and a pink color occurs when Serratia marcescens is plated on an Eosin Methylene Blue plate. Upon entering the lab, I discovered metallic green colonies on the plate. I then knew I had either Escherichia coli or Citrobacter freundii. To find out which I chose to inoculate a tryptophan broth with my…

the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either ampicillin or not as well as with a ribosome known as arabinose. The E. coli without pGLO died in the ampicillin and the ones with pGLO survived…

Transforming Genetic Material To E. Coli Using Plasmids Proteins are created within the body through a process known as central dogma. This process is the transcription of DNA to RNA and the translation of RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its…

manipulate gene sequences in DNA. E. Coli was the bacteria that was first used to accept the plasmid known as pSC101 which was resistant to certain antibiotics (Genetics and Genomics Timeline). After repetition of this new process they decided to use the genes from the Xenopus laevis toad into E. Coli bacteria. They saw that the bacteria reproduced at an astonishing rate and recorded that the genetic material from the toad passed through different generations of E. Coli bacteria. Soon after…