Escherichia coli O121

Malabanan, Joshua Yran P. Professor Cynthia Solem English 1A 05 December 2014 How E. Coli Can Make Us Die A 4-year-old Oregon girl has died from medical complications possibly resulting from an E. coli infection contracted sometime just before Labor Day weekend, according to the girl’s uncle, who spoke with Food Safety News. Serena Profitt of Otis, OR, died Monday at Doernbecher Children’s Hospital in Portland. Her symptoms of E. coli infection first appeared around Aug. 29 with bouts of diarrhea, said Travis Profitt, Serena’s uncle. A spokesman for the Lincoln County Health and Human Services Department said that health officials are not ready to announce a cause of death because they have not completed all laboratory testing on the girl’s…

acid is particularly good at “shuttling” protons, or acidity, across cell membranes. Once inside the cell, there are four broad acting mechanisms that inhibit the bacterial cell. Lactic acid disrupts cell regulation on a general level, it causes bacteria to spend energy to maintain ph. by pumping out acid, causing the bacteria to change their metabolism to produce alkaline metabolites and acid stress generates free radicals that damage all cellular mechanisms9. However, Ajax Spray N Wipe does…

The purpose of the pGLO transformation lab is to teach students how to perform genetic transformations. According to Bio-Rad’s lab manual, genetic transformations is process of inserting “a gene into an organism in order to change the organism’s trait.” In this particular lab, students will be taking the pGLO gene, which makes the protein Green Fluorescent Protein (GFP), commonly found in a species of jellyfish, which glow (N.d., p. 32). Theoretically, when the pGLO gene is inserted into the E.…

Unknown #30 1a. Glucose fermented. 2a. Sulfur reduced. 3a. Lactose fermented…………………………………………Citrobacter freundii 3b. Lactose not fermented 4a. Sucrose fermented…………………………………….Proteus vulgaris 4b. Sucrose not fermented………………………………Proteus mirabilis 2b. Sulfur not reduced. 5a. Lactose fermented. 6a. Sucrose fermented. 7a. Ornithine decarboxylase present……Enterobacter aerogenes 7b. Ornithine decarboxylase not present…….Klebsiella pneumonia 6b. Sucrose not…

step of the catalytic reaction occurs when AP binds to a phosphomonoester to form an enzyme-substrate complex (E*DOP). When the enzyme is phosphorylated, it forms a covalent enzyme-phosphate complex (E-P); hydrolyzing the E-P complex will give noncovalent E*P (6). This process results in an inorganic phosphate and an alcohol. As suggested by Kim and Wyckoff, if a high concentration of phosphate acceptor is available in solution, the enzyme would transfer the phosphate group to an alcohol (6). …

agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test using a peptone-iron agar deep. The results of the H2S production test showed no production of H2S near the inoculation point which meant that my next test performed would be the MR/VP test using MR/VP broth. The MR test…

The role of this structure in the pathogenesis of the bacteria is of high medical importance as it helps the bacteria to be propelled or display from one location to another carrying the infection to other areas fast, an example is the Escheria Coli, which can propel from the urethra to the bladder, known as the most common causes of urinary tract infection (UTI’s), and some other as flagellum cannot be seen under microscope without stain, helps to differentiate them using specific antibodies…

Due to limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral…

These results lead to the identification of unknown 18X as Escherichia Coli. The Gram test was first performed on the provided culture of unknown 18X. To double-check the results, a second Gram test was performed from the incubated nutrient broth that was grown from a colony of the streak plate. It provided the same results as the first test, Gram-negative bacilli. Since, unknown 18X was determined to be a Gram-negative bacillus that grows best at 30°C, seven of the potential thirteen…

protein contamination; ideally, the ratio should be between 1.8 and 1.9, denoting the relative absence of protein contamination. Because DNA does not absorb light at 320 nm, a pure sample of DNA should have an absorbance at 320 nm that is less than 5% of its absorbance at 260 nm. 230 nm is the minimum absorbance of DNA; a high value at 230 nm indicates contamination with aromatics and salts (Arruda et al., 2013). The 260/230 ratio should ideally be between 1.8 and 2.2. A result below 1.8 would…