Equilibrium

Aim: This is an investigation to establish the effect of different temperatures (from 0°C -100°C) on the production of oxygen gas in a potato catalase reaction, using hydrogen peroxide as a substrate. The oxygen gas produced will be measured using a gas pressure sensor. Introduction: Enzymes are proteins, known as biological catalysts that increase the rate of reaction in a biochemical reaction without being chemically altered. The human body consists of various different types of enzymes,…

create equilibrium? Hypothesis: If the differences in masses (grams{±0.1g}) on each side of the pulley increases, then the mass in the middle of the two pulleys will be required to increase, because they will have to balance out each other’s mass to establish equilibrium. If the mass (grams{±0.1g}) on a side of a pulley decreases, then the mass in the middle of the two pulleys will be required to decrease, because they will be required to balance out each other’s weight to establish…

Energy needed for life comes from the breaking or forming of chemical bonds during chemical reactions. Energy is released in the formation of bonds and energy is also released when the bonds are broken, but in both energy is required to start the chemical reaction. This energy needed to start a chemical reaction, activation energy, comes from enzymes. Enzymes (almost always proteins) are organic catalysts that help speed up the rate of reactions and allow the reactants to get the most out of the…

The aim of this experiment was to investigate which technique – out of gravimetric analysis and colourimetry – was the most appropriate and accurate method to practice when calculating the percentage yield of a reaction. When examining the data obtained from the investigation, there was evident that there were some trends in the results. For the three graphs showcasing the results for gravimetric analysis in task one and task two, there was no general pattern. This was due to the fact that in…

Potato Bubbles Introduction Our driving question for this lab was “How do changes in temperature and pH levels affect enzyme activity?” Throughout the duration of this lab, we explored enzymes and how environmental factors like pH and temperature affected enzyme activity. Enzymes are proteins that speed up chemical reactions. For this particular experiment, we studied the enzyme catalase, which is found in potatoes. Catalase reacts with hydrogen peroxide to break it down into its water and…

6. Quantitative determination of rat peroxisome proliferators activators γ (PPAR-γ): Rat PPAR-γ concentration was determined by ELISA; using a commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer…

The Michaelis constant, Km, is the amount of substrate needed for the enzyme to obtain half of its maximum rate of reaction. (Oregon 2015) It reveals the enzymes affinity for the substrate and shows the magnitude of the enzymes affect on changing the rate of the reaction. For example, an enzyme with a high Km needs a large concentration of substrate to efficiently increase the rate of the reaction. (Oregon 2015) A low Km indicates that the enzyme will increase the rate of reaction with a small…

FORCE AND STATIC EQUILIBRIUM Introduction The research is to be made on the most important concepts of the civil engineering which are force and static equilibrium. These concepts are very important for the engineering point of view. The force can be considered as the element which moves or tries to move and stops or tries to stop the body in motion. So whenever there is a force applied on an object it changes its inertia and causes to changes its state of rest or motion. The structures should…

Introduction An enzyme is a protein that functions as a catalyst, it speeds up chemical reactions (Freeman et al. 2017 p. 90). Enzymes are often large globular proteins and are able to hold substrates in specific orientations so they are able to react, the location where the substrate binds with the enzyme and reacts is called the active site and is the location where the catalysis occurs (Freeman et al. 2017). Enzymes activity is often related to the optimal environment for them in respect to…

Abstract: In this lab, three variables were tested to discover their effect on the enzyme catalase, taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that…