Enzyme

IRRADIATION Food irradiation is the processing of food products by ionising radiation in order to control food borne pathogens, reduce microbial load and insect infestation, inhibit the germination of root crops, and extend the durable life of perishable produce (International Consultative Group, 1991). Application of ionizing radiation results in the radiolysis of water. This triggers the development of species such as OH−, hydrated electron and H+, which can then induce several chemical…

and dibasic potassium. Phosphorus occurs naturally in every cell in the body and is used for repair and growth of cells and tissues. As an organic and inorganic phosphate, it has a significant biochemical function in the body creating metabolic and enzyme responses in almost all organs where it influences the steadiness of calcium levels, neutralizes acidic and basic ions in a solution and has a role in…

Bovine serum albumin (BSA) is usually used for generation of nanoparticles (NPs) in drug delivery systems. This paper presents a study on the simple coacervation method for fabrication of BSA nanoparticles.An artificial neural network (ANN) model was developed to predict the diameter of nanoparticles based on experimental data. Effect of pH, temperature, initial BSA and cross linker concentration, molar ratio of BSA to ethanol, agitation rate and rate of ethanol addition were investigated on the…

We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…

The proximate composition of purple soymilk (Table 1) is not much different from that of yellow soymilk. Purple soymilk has protein, ash, fat, carbohydrate and moisture content 2.76 ± 0.13, 0.12 ± 0.08, 1.17 ± 0.06, 1.27 ± 0.10, and 94.69 ± 0.04% respectively, while yellow soymilk from Tunde-Akintunde and Souley [35] has a protein content of 2.23-3.05%, ash content of 0.24-0.32%, fat content of 1.60-1.94%, carbohydrate content of 1.99-2.78% and moisture content of 92.02-93.29%. Black soybean…

been caused by a liver dysfunction. According to Thepa (2007), serum bilirubin is elevated when there is an impairment in the excretion of conjugated bilirubin from hepatocytes of bile ducts. The results also showed elevation of two of the liver’s enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), these elevation could be caused by impaired liver function (Pratt et al, 2000). Mariana’s serum ALT levels is higher than the serum AST which could be caused by a form of…

Purpose The purpose of the experiment was to investigate the effect of the amount of drops of lemon juice added to skim milk on the mass of the formed curds. Background Information Milk curd is defined as the byproduct of the effects of acid and enzymes on milk protein and fat (Milk Chemistry). Fresh cows milk has a pH of between 6.7 and 6.5. When milk is acidified, the fat and proteins begin to curdle, or coagulate, causing the milk to separate into curds and whey. Curd contains most of the…

acetylcholine and transmit a message to the next neuron (Waymire, n.d.). However, in order for a neuron to return to its initial, unstimulated state, the acetylcholine in its receptor must be broken down into acetyl and choline. Cholinesterase is the chief enzyme that is responsible for the reduction of acetylcholine (Waymire, n.d.). Because cholinesterase is inhibited upon the application of carbaryl, acetylcholine is prevented…

Discussion on isolation of protein from spinach leaves In this experiment, we tried isolating an actual protein called Rubisco from spinach leaves. The full meaning of these Rubisco means Ribulose-1, 5-bisphosphate carboxylase/oxygenase which is an enzyme that catalyzes the addition of a carbondioxide group to ribulose-1, 5-bisphosphate during sugar production in green plants. Although Rubisco is an abundant protein in plants, it is not the major component in all spinach cell and to successfully…

aureus consist of a polyribitol-phosphate backbone of 11-40 repeating units(Xia10) decorated with three tailoring modifications: D-alanylation, a-O-GlcNAcylation and B-O-GlcNAcylation(Xia,Swaboda).Prior to this Brown’s work, most of the genes and enzymes involved in WTA synthesis and decoration had been identified, through genetic and in vitro reconstitution approaches(swoboda,xia). Although…