Enzyme

3.1: Ligand preparation: A series of benzimidazole derivatives were used to prepare the ligand dataset using benzimidazole as the query molecule. All the inhibitors selected comply with the Lipinski rule of five. A total of 50 molecules were taken up for the study. 3.2 Virtual screening: To further screen the compounds, additional similarity metrics were calculated by ROCS. ROCS is a shape based superposition method used to perceive similarity between molecules based on their three dimensional…

Abstract: Fermentation of sucrose with brewer yeast catalyst was successfully conducted to yield ethanol with 70.2 % conversion of sucrose. Based on the graph of Refractive Index vs % ethanol (v/v), 32.8% of ethanol was determined to be in the distillate solution with corresponding Refractive Index of 1.3555 Statement and purpose The purpose of this experiment was to determine the % of ethanol (v/v) in the resulting solution and the % conversion of sucrose Introduction The fermentation 17.1 g…

Summary: Dopamine is a neurotransmitter that activates different dopamine receptors throughout the central nervous system. As mostly invertebrates have been studied in the past, the researchers wanted to focus on vertebrates in this study in order to determine the evolutionary patterns of these receptors. Five dopamine receptor genes were studied in five different mammals, and it was discovered that genes that are expressed more broadly have slower evolutionary rates than genes that are…

to determine reducing sugar content of food sample by performing Fehling method and to identify invert sugar content of sugars by using polarimetry and also to learn property of invert and reducing sugar. Carbohydrases represent a large class of enzymes hydrolyzing polysaccharides and low-molecular-weight glycosides. They belong to the class of hydrolases. Carbohydrases are classified according to their specificity…

Francisella tularensis is a bacterium that has been recognized to be an agent responsible for the disease known as tularemia. This disease, in essence, sends the immune system of the organism into a hyperactive state, sometimes referred to as hypercytokinemia due to the elevated levels of various cytokines, which if not treated properly could lead to extremely high fevers, inflammation of various tissues, and (if contracted through aerosol form) the death of the infected organism. Specifically,…

The Enzyme Electrophoretic Separation of Proteins lab experiment will take place on March 7th, 2017, from 2:00pm to 5:00pm. It is important for students to follow lab safety guidelines in order to prevent any complications that may be a risk to one's health. For this experiment, students must wear their lab coat, goggles, and gloves at all times. All materials in this experiment should be handled with caution to avoid any damages. Students must keep hands away from face, eyes, and mouth, when…

RESULTS Phytic Acid The concentration of phytic acid was determined spectrophotometrically in three tested plant materials of Gishta as shown in table 2. The concentrations of phytic acid were obtained from 5 g dried leafy material. All the leaf samples contained phytic acid (detection limit 0.025 mg/ml) in this study. This may have been due to the fact that there was a limitation in terms of the detection concentration since concentrations less than mg was not tested. Seed extracts was found…

Almost every cell goes through a phase called the Cell Cycle this is where they cell grows and develops and then eventually splits into two cells. Why is this important? Well through the cell cycle it repairs damaged cells or replaces worn out cells. When you break your leg the cells work to get the bone healed or when you have a cut the cell cycle will help you by dividing cells to create a scab for your cut to heal. The cell spends most of its life in interphase, then it goes to the mitotic…

First, adding 2ul of 5 times gDNA eraser, 3ul of DEPC water and 5 μl of RNA solution into PCR tube, then heated at 42 ° C for 3 minutes in an ABI PCR machine and then placed on ice for a while. Adding 2 μl of RT buffer, 1 μl of RT enzyme mix, 2 μl of RT primer and 5 μl of DEPC water into PCR tube, then heated to 42 ° C, 15 minutes, 95 ° C for 3 minutes and placed on ice for a 1 hr. cDNA was divided into three groups, one group was positive viral RNA, negative viral RNA, and internal…

distribution of these elements is dependent on brain region, subcellular location, age, species, genetic and environmental factors (Prohaska, 1987). These elements are considered as cofactors, catalysts and structural components for many cellular enzymes and proteins in the CNS. They also play roles in the homeostatic, regulatory mechanisms maintaining their concentrations in the CNS (Richardson and Ponka, 1997). They take part in neuronal activities such as neurotransmission, synaptogenesis,…