Enzyme

Phosphonates as inhibitors for Pks13-TE Hypothesis for the aim 1 of the proposed project is that the fluorinated α–Aminophosphonates as a covalent irreversible inhibitor for the Mtb Pks13-TE by scission of the P-C bond. Previous studies have shown the fluorinated aminophosphonates (FAPs) demonstrated serine esterases inhibition via scission of the P-C bond as shown in Figure 2.1.A 4. FAPs are known to irreversibly inhibit serine esterases such as acetylcholinesterase16 , neuropathy target…

The data obtained from the spinach disks experiment was inconclusive. Sample one had 50mL of distilled water at a temperature of 22°C, it took the spinach disks an average time of 17:06 minutes before reaching the top. This occurred because the enzymes in the spinach disks were functioning around an optimum temperature for photosynthesis and the production of oxygen. Sample two had 50mL of distilled water at a temperature of 10°C, the spinach disks had no movement at all. Sample three had 50mL…

increase, the kinetic energy where H2O2 molecules bind frequently with the catalyst increase the enzymes. Conclusions: An enzyme speeds up the chemical reaction, the activation energy allows energy to require to break bonds apart and lower activation…

break hydrogen peroxide into oxygen and water the faster that it could speed up chemical reactions in the bodies or organisms. The results, at first may seem dry, but they demonstrate how crucially important it is we know what the optimal pH is for enzymes like catalase. This knowledge allows us to understand better our bodies, and the chemical reactions inside it, as well as enable us to protect the systems that we depend on to stay…

mesophylls and grow at moderate temperature.These values are called cardinal values. Temperature range of different enzymes are specific and it varies with different organisms.Enzymes are very sensitive to temperature variations and denatures at the higher temperature.Organisms show higher temperature range…

of catechol oxidase used from a solanum tuberosum specimen Abstract The various effects of temperature and pH on the enzyme activity and action of catechol oxidase, an enzyme containing copper which oxidizes phenols such as catechol with the use of dioxygen, were examined throughout several trial procedures. The experiment was conducted to determine the optimal pH for an enzyme to carry out its action, as well as to determine…

B there was no color change, thus the microbes do not reduce nitrate. For the hydrolytic test, the starch plate had highlights around the bacterial growth indicating starch hydrolysis. In the fat containing plate there was no dark pigment, meaning enzyme wasn’t made. The casein test had no clear zones, meaning casein wasn’t degrade. For the Tryptophan degradation, no red was detected on top of the medium, which indicated that it was negative. Urea hydrolysis test was also negative no color…

Chapter 4 - Discussion A standard curve of p-nitrophenol was drawn at the start of the project, before any enzyme assay of the fractions. The standard curve is set up so the equation of the line can be used to measure the enzyme activity in each sample fraction. P-nitrophenol shows a linear relationship with acid phosphatase. An Enzyme assay gives the activity levels of acid phosphatase in each fraction. The results from each fraction were placed into the equation of the line from the standard…

Introduction: Enzymes play a major role in biological systems to catalyze and control different reactions. The study of enzymes’ behaviors toward substrates and products is significant to understand various enzyme catalyzed reactions in living organisms. One of the most important enzymatic group is cytochrome P450 (CYP450) which contribute to the metabolism of many endogenous as well as exogenous substances such as drugs. Although there are several subtypes of CYP450 family, CYP450 1A1 is going…

Simultaneous hydrolysis and fermentation (SHF) of sweet sorghum juice is a multi-step process and interactions between enzymes inside the yeast and the complex substrate. In addition, SHF also undergoesmechanisms by the product and other components inside the substrate. The process, interactions, and as well as inhibition mechanism of SHF are not fully understood. A modified kinetics model based on those reported by Philippidis [24] and Shadbahr [17] were used in this study to quantify the…