Enzyme

extracellular fraction from a stationary phase culture, to test the localization of the enzymes. For the protease assay, a specific soluble chromogenic substrate called azocasein was employed to determine presence of enzyme activity. The degradation of this substrate in trichloroacetic acid produces a colour change that can be registered using a spectrophotometer, and this change in absorbance over time is proportional to enzyme activity (Charney and Tomarelli, 1947). We measured the absorbance…

1. Introduction Hydroxynitrile lyases (HNL, EC 4.1.2.x) are enzymes which mediate the release of hydrogen cyanide (HCN) and aldehyde or ketone from cyanohydrins [1]. These HNL catalyse enantioselective synthesis and cleavage of cyanohydrins [2]. The cyanohydrins and their derivatives are finding wide applications in industries like pharmaceuticals, agrochemicals and cosmetics since these can be readily converted into other important molecules like α-hydroxy carboxylic acids, α-hydroxy ketones…

Immunocytochemistry (ICC), which this lab is based on, is commonly used as a laboratory technique to anatomically observe the location of a specific protein or antigen in cells by the use of a specific primary antibody that binds to it. A staining reagent or chromagen is used to help detect certain proteins used in this experiment, called 3,3'-Diaminobenzidine (DAB). 3,3'-Diaminobenzidine (DAB) DAB, a derivative of benzene, is an organic compound, which is both chemically and thermodynamically…

multi copper oxidases (Mate and Alcalde, 2016a).They catalyze the oxidation of compounds that are coupled with the four-electron reduction of "O" _"2" "to" "H" _"2" "O" . They are regarded as eco-friendly enzymes. Laccases have been first discovered in 1883, which makes it one of the oldest enzymes ever observed (Kunamneni et al., 2008).Laccases are found in a diversity of fungi, bacteria and higher plant species (Santhanam et al., 2011). These laccases are involved in a degree of…

In this lab experiment, we set out to test the efficacy of the enzyme Amylase in different pHs and heat. The objective of the experiment was to prove that the enzyme Amylase is the least efficient in a higher or more basic pH. According to the data, the hot solution of amylase, most likely due to the UV rays. The wavelength of the hot solution was 0.6696nm, significantly smaller than the other solutions. The base, which was predicted to be the least effective came in at 2nd least effective at…

Introduction Lactate dehydrogenase also known as LDH is a critical enzyme that is found in all mammals. It exists as a tetramer in five isoforms with a combination of heart and muscle subunits. One of its main functions is to catalyze the reaction of pyruvate to lactate. This reaction occurs in an anaerobic environment where the body needs to make sufficient energy as fast as possible, therefore pyruvate ferments to lactate with the help of LDH to produce enough NAD+ to power glycolysis. LDH-5,…

Hypothesis: Aerobic Respiration: If a phosphate buffer, dichlorophenol-indophenol and succinate are added to a mitochondrial extract then the succinate molecules will bind to an enzyme complex (succinate dehydrogenase). Because the enzyme and FAD are a part of the same complex, initiating succinate oxidation, this reaction reduces FAD to FADH2. Thus the oxidation of succinate to furmarate. Anaerobic Respiration: If a solution of yeast and glucose is combined and placed in different incubation…

Coli is therefore no exception. Those enzymes carry out many different functions in the cell and are of vital importance, so their pH sensitivity puts cells at a great risk when confronted by a change in pH. This is due to the covalent bonds between amino acids that have a positive charged part…

Gene Expression Paper RESULTS The hypothesis is that as the amount of lactose increases in a treatment, the higher the enzyme production and activity. A total of six treatments were done in order to determine whether the amount of lactose in a substance correlates with the amount and production of b-galactosidase. Experiment 1: The use of OD600 as an indicator of bacterial population size. In addition, the use of Abs 420 as an indicator of protein production. Then the Miller units of…

Enzymes speed up the rates of most biochemical reactions by helping reactants interact with each other. Since enzymes are highly specific for a specific reaction, they only catalyze one or a few types of reaction. One of important enzymes in human body and many other animals is amylase that hydrolyzes starch, a major part of human diet for many people in the world. There…