Enzyme

Specific environmental conditions may play a role in how enzyme activity is affected. We conducted an experiment to observe whether the change in temperature altered the reaction rate of an enzyme. The activity rate of our enzyme, catechol oxidase, was collected by analyzing the changes in absorption rate of the enzyme in a buffered catechol solution after being placed in four different temperatures. In which one solution remained out in room temperature being to be our negative control. Each of…

After the experiments we did in class, I came to the conclusion that when starch is in the mouth, enzymes in our saliva break it down and turn it into glucose. My first piece of evidence comes from the investigation where we chewed a cracker with starch for two minutes. As we chewed, the cracker’s taste changed from bland to sweet. As we chewed, the enzymes in our saliva broke down the starch molecules, which caused them to rearrange and create a new product: glucose. My second piece of…

count-1025 Background research Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all other proteins have secondary and tertiary structures which are affected by temperature, pH and other factors. Due to the fact that enzyme function is related to the structure of any enzyme; enzymes are heavily affected by…

There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as…

substrate concentration (%N × 6.25), (ii) enzyme-substrate ratio (E/S) in % or in activity units per kg N × 6.25, (iii) pH, (iv) temperature, and (v) time (Adler-Nissen, 1984). Generally, there is an optimum combination of both pH and temerature, where the enzyme shows the highest activity. Physicochemical conditions of the hydrolysis reaction such as temperature, pH and enzyme/substrate ratio must be adjusted to optimize the activity of the proteolytic enzyme (Kim & Wijesekara, 2010; Santos,…

pH will affect enzyme function by increasing the reaction rate because as temperature increase so is the rate of reaction and extremely high or low pH will result in the denatured by decreasing the reaction rate. The function of this experiment was to investigate the influence of the concentration of enzyme on the rate of reaction. The rate of reaction in this experiment was subjected by the amount of Guaiacol, H2O2, Extracts, and Buffer (pH5). The reaction rates with different enzyme…

Assessment of enzyme producing efficiencies of endophytic fungi Extracellular enzymes assay was conducted to investigate the production of enzymes by the endophytic fungi Amylase activity Amylase enzyme was assessed by growing the fungi on glucose yeast extract peptone (GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium…

I. Introduction Catalysts are stimulus that speed up the rate of a reaction by lowering the activation energy of the reaction. Chemists discovered catalysts when they were trying to determine how to turn one substance into another. Although early chemists did not resolve how to turn substances into one another, they did discover that various materials could be changed from one form to another. This also led to the discovery that if certain chemicals were present in the reaction, the change would…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

this series of experiments the productivity and function of the enzyme Alkaline Phosphatase will be examined. Alkaline Phosphatase is an enzyme commonly produced and found throughout the entire human body. Its main function is the cleaving of phosphate groups from mostly organic molecules, including proteins, nucleotides and even alkaloids, which makes it a hydrolase enzyme (Ellis, 2016). In biochemistry, a hydrolase enzyme is an enzyme that speeds up the hydrolysis of complex biomolecules like…