Enzyme

Catalase Enzyme Lab Write-Up Our goal with this lab is to discover how enzymes affect the production and destruction of harmful chemicals our body makes into harmless chemicals. Our body contains hundreds of enzymes in our cells and this lab will show us how they interact with parts and materials of our bodies. The enzyme we will be focusing on is called catalase. It’s job for the body is to break down hydrogen peroxide a harmful substance into two harmless substances oxygen and water.…

Effects of Temperature and Substrate on Catalyst Enzymes Ryan Smith Lab Section 030 Abstract In order to turn hydrogen peroxide into water and oxygen by using enzyme called a catalyst. To determine the effects of temperature and substrate concentrations we preformed an experiment testing the effects on the catalyst enzyme reaction. The hypothesis for our first experiment was if the substrate concentration is larger than the catalyst enzymes activity will be faster as well. The result followed…

Boronic acids have attracted attention as an alternative receptor to enzymes for saccharide detection (e.g., glucose oxidases for glucose detection).58-60 The enzyme-based sensing has the disadvantages that, since it is based on the rate of the reaction between the enzyme and the analyte, this approach is sensitive to various factors that affect the enzyme activity and the mass transport of the analyte, it consumes the analyte, and it requires mediators; in contrast, the boronic acid-based…

Abstract: An enzyme, a protein, is a catalyst, which increases the rate of the chemical reaction. The results of this experiment showed how different temperatures could affect the chemical reaction between the enzyme and the hydrogen peroxide. The temperatures tested in this lab were 30, 35,40,45 Celsius, which are close to the average body temperature which is 37 Celsius. Catalase is an enzyme which breaks down hydrogen peroxide into oxygen and water. Different temperatures were measured to see…

The rheology characteristics of enzyme clarified beetroot juice with added additives such as maltodextrin (MD), inulin (INU) and their combination (MD+INU) was studied at a wide range of concentrations (0 to 20% dry solids) and temperatures (5 to 80°C) by controlled stress rheometer. The rheological parameter shear stress was measured upto the shear rate of 600s-1. The temperature dependency of Newtonian viscosity of beetroot juice was described by Arrhenius equation. The effect of added solid…

study was to see how enzyme activity would change as there was a change in temperature and pH. Both temperature and pH were hypothesized to have an affect on enzyme activity. Each had an optimal temperature. At this point, the rate of enzyme activity would stop increasing and begin to decrease. For temperature, it was hypothesized that the optimal temperature would be 48°C. For pH, it was hypothesized that the optimal pH would be 7. As temperature was increased, the enzyme activity also…

Pectinase is one of the widely used enzymes in the food and enzyme industry. It is an enzyme group that comprises polymethylgalacturonase (PMG), polygalacturonase (PG), and pectin lysase. These enzymes are used in degradation of pectic substances, which involves deesterification and depolymerization reactions. Pectic substances are the major structural component found in plant’s cell wall and lamellae. Types of pectic substances include protopectin, pectin, pectinic acid, pectic acid and…

experiment the students studied how warm water affects the yeast, and learned that warm water activates the yeast enzymes. Where cold water may denature the enzyme, as the students previously reviewed that three factors that affect enzyme activity are temperature, concentration of enzyme, and pH level, and if any of these three factors were to exceed out of the specific range, the enzyme will denature. This would be compelling to test because the students will then see how the cold water affects…

The time required for the filter paper to float reflects the enzyme movement immensely. The enzyme movement is slower when there is a greater amount of time for the reaction to occur. As the temperature increased, the reaction time was quicker. For example, the class average for the substrate temperature “Hot” was 7.28 seconds, which was a drastic change from the temperature “Cold” of 66.54 seconds. The reason behind the change was that heat makes molecules move quicker, and cold makes molecules…

CYP2D6 is a Phase I oxidative metabolism enzyme that is clinically important because about 20-25% of clinically used drug are metabolized by the CYP2D6 enzyme. CYP2D6 substrates are typically lipophilic and include drugs such as antidepressants, antipsychotics, antiarrhythmic, antiemetic, and opioids. The exact clinical effect of the polymorphism is dependent on the drug and the variant alleles expressed because individual allele variants can lead to a range of metabolic ability from ultrarapid…