Enzyme substrate

In a biochemical reaction, enzyme which are proteins based compounds are used as a catalyst to produce a high yields. Proteins are the macromolecular components of almost enzymes, except for ribozymes, a class of RNA modifying catalysts. Enzymes are classified on the basis of their composition; simple enzymes are those composed wholly of proteins, whereas, complex enzymes which also known as haloenzymes, composed of protein plus a relatively small organic molecule. In this terminology the…

Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of the chemical reactions that take place within cells. They are vital for life and serve a wide range of important functions in the body, such as aiding in digestion and metabolism. Some enzymes help break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are highly selective…

there for proteins are broken down into amino acids and then go through the anabolic reaction which builds them back up 8) Would you hypothesize that the meat tenderizer would still work if it were? Heated? Why? Meat tenderizer is an enzyme. Enzymes denature in extreme heat but work really good in room temperature 37c. So it depends what the temperature is. if it’s really hot around 60c Then it won 't work but if it 's room temp around 37 c then it will work. Liver Lab Analysis –…

the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg respectively. Fig. 4. Determination of Michaelis constant of GCase for different p-NPG substrate concentrations…

Abstract: Catalase is an iron porphyrin enzyme present in high concentrations in humans and animals liver tissues. It is a peroxisomal enzyme that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate. Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site…

In non-competitive allosteric inhibition, inhibitor molecules bind to an enzyme at the allosteric site. Their binding induces a conformational change that reduces the affinity of the enzyme's active site for its substrate. The binding of this allosteric inhibitor changes the conformation of the enzyme and its active site, so the substrate is not able to bind. This prevents the enzyme from lowering the activation energy of the reaction, and the reaction rate is reduced. However, allosteric…

energy required. An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. Enzymes carry out the thousands of chemical reactions that occur on cells, or the basic units of structure and function of an organism. Usually, they are polymers of thousands of amino acids. Enzymes are not used up as it carries out the reaction but are recycled over and over. In this experiment, peroxidase from horseradish was the enzyme used to test…

of the reaction it is causing. The experimenters will use pieces cut up potato as an organic catalyst. The potato contain enzymes that will perform the reactions. Enzymes, “are proteins, produced by living cells; they catalyze chemical reactions by lowering the activation energy. Activation energy is the energy required for a chemical reaction to occur” (Carter 1). Substrates or reactants are the molecules that are…

Immunocytochemistry (ICC), which this lab is based on, is commonly used as a laboratory technique to anatomically observe the location of a specific protein or antigen in cells by the use of a specific primary antibody that binds to it. A staining reagent or chromagen is used to help detect certain proteins used in this experiment, called 3,3'-Diaminobenzidine (DAB). 3,3'-Diaminobenzidine (DAB) DAB, a derivative of benzene, is an organic compound, which is both chemically and thermodynamically…

indicates a constant enzyme reaction rate. The activity rate of catalase in figure 1 is calculated to be approximately 0.15mL/sec. The following figures show how this rate of reaction is affected by different external factors. The results in figures 2 and 5 show an increase in either enzyme concentration or substrate concentration will increase the enzyme activity. In figure 2, the 0.75% enzyme concentration had the highest enzyme activity with a rate of 0.11mL/sec; whereas the 0.25% enzyme…