Enzyme substrate

In the last two weeks, Roundup was added to water and the spectra were measured. The initial test ranging from 1% to 100% was to concentrated. This was indicated because the spectra’s absorbances were larger than one. Furthermore, when looking at figure 1, once above 0.30% data flattens off. Once the range was narrowed with serval more trial the range being now sampled is between 0.01 % to 0.30%. The lambda max is ranging from 307 to 309 wavelengths. Glyphosate’s structure contains three major…

By “inactivating enzymes, alteration of drug targets and alteration of the ability of drugs to enter and or accumulate in its cells”, Enterobacter aerogenes has an interesting way of combating drugs (Enterobacter Aerogenes). This organism lives in “the endogenous human gastro-intestine”…

The method of this investigation is based around how much optimal water is needed in order to maximise efficiency plant growth. 1. Collect four petri dishes and place even amounts of cotton wool balls into each of the four petri dishes 2. Place 20 plant seeds across the four petri dishes. That means there should be 5 seeds for every petri dish. Make sure that the 5 seeds are located in different positions on the cotton wool. 3. Place 100 ml of water into a beaker. 4. You will have four petri…

Labatt’s Brewery is looking to find a temperature sensor which can withstand the conditions of the fermentation processing which occurs in its company. The sensor must be able to operate under a large temperature range (-2C to 200C), while maintain the temperature with at least < +/- 0.5°C accuracy. It should also have resilience to contact with hot caustic NaOH, as a flushes occur between batches in order to clean and sterilize the equipment. Furthermore, it should be non-contact sensor as…

Arthur Peter Yagubyan Bio 360 Genetics 10/08/15 Malone Effect of the consumption on buriti oil on the metabolism of rats induced by iron overload a) What was the hypothesis of the figure? b) What question(s) did they ask to address the hypothesis in the figure? c) What did the study do in this figure (NOT overall paper)? d) How did they do it (in this figure)? e) What did they find in this figure? f) What did it mean in this figure? Figure 2. a.) The…

Stromelysin-1 Stromelysin-1 is used to identify risk of developing CAD. Stromelysin-1 or matrix metalloproteinase-3 (MMP-3) is a matrix remodeling enzyme which degrades collagen, proteoglycans, gelatin and elastin. Therefore MMP-3 affects the matrix composition, which affects lipid accumulation, vascular remodeling and plaque instability resulting in cardiovascular disease. Human MMP-3 gene is found…

The ΔglgC cyanobacteria will be transformed yet again to express an engineered new, small PSII antenna. In order to do this, plasmids pGDNG3/Cys(181/226)Ser and p613 will be co-transformed using the method described in the paper by Beckman et al. The method of using a vector containing an antibiotic (ampicillin) resistance gene will also be employed in this experiment. Ampicillin will then be added to the transformed bacteria in order to obtain the successfully transformed bacteria. By adding…

More conjugated compounds absorb higher wavelengths/lower energies because as the wavelength increases the HOMO/LUMO gap (ΔE) decreases which is due to the compression of multiple molecular orbitals (ψ). According to Klein, the Ketchup stain (lycopene) on a shirt and upon application of bleach (NaClO), it breaks lycopene’s conjugation, therefore causing a decrease in conjugation; which directly correlates to the stain no longer being visible to the eye but when illuminating the shirt with…

Proteins are a multifarious group of macromolecules, which carry out many different functions within the human body. (Mbuthia, 2009). They are comprised of repeating units of amino acids held together by peptide bonds. As stated by Mbuthia, the Biuret reagent is a solution which aids scientists to conclude if there are any peptide bonds present within a sample, and thus, is used to determine protein existence within a substance. The Biuret assays depends on the presence of 2 or more peptide…

Introduction Phospholipase A. is an enzyme used by Leukocytes to destroy bacteria, by causing membrane permeability. In multiple purification steps, we must isolate the enzyme to be able to conclude it can be used for antibiotic use. Using homogenate, acid precipitation, ion exchange chromatography, and 2 from of gel filtration, the purification will be tested. I hypothesize these purification steps will isolate and purify the enzyme desired. Results Figure 1 shows Sephadex carboxymethyl ion…