Enzyme substrate

Introduction Enzymes are specialized proteins present in all living organisms, whether animals, plants or microorganisms (Reece et al., 2012) .In addition, life would cease to exist without these vital organic molecules, as they are needed for metabolic processes, and other reactions and processes in the body (Reece et al., 2012). Sometimes some RNA molecules can act as enzymes (Reece et al., 2012). Enzymes as well as their substrates work best at optimal levels, and are affected by temperature…

The effect different temperatures have on the rate an enzyme works was tested by this lab. The concentration of the enzyme catalase in different types of cell was also tested. Enzymes are balls of protein that change a specific substrate into a product by contact at the active site. Enzymes are reusable and once they finish tuning one substrate into a product, it moves on to the next substrate it bumps into in just the right way. The enzyme catalase, which this lab focuses on, can be found in…

from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar concentration and volume in place of substrate information. The inhibitor concentration was used along with the slope of series B to find Ki. A sample calculation is shown below. Then the same steps were…

molecules that rise over the activation energy level the faster the reaction occurs. Catalysts lower the activation energy level allowing the reaction to occur more rapidly (Barnhart and Hopper 2011). Most catalyzes are also known as enzymes (Barnhart and Hopper 2011). Enzymes are designed to only be able to…

Enzymes are important substances made by the cells of plants and animals. There are called catalysts, which are substances that control how quickly chemical reactions occur without interfering at the reaction products (Daja and Treska 2015). Structure of both the enzyme and substrate, the substance on which an enzyme acts; provides clues as to how an anticipated reaction can be favored by the alignment of catalytic residues (Johnson 2008). The reaction of an enzyme is represented as: In…

to NAD+ by CMI(9) . By coupling the associated metabolisms of these two enzymes NADH is produced using light and water as substrates with oxygen as the only…

Lactose is a sugar found in milk and milk products. Lactase Is an enzyme found in the small intestine that breaks down lactose into simple sugars such as glucose. The substrate has to fight to bind with the enzyme lactase. The inhibitor tries to attach to the lactase and slow down the chemical reaction while the substrate does the opposite. The substrate increases the rate of the chemical reaction, this chemical reaction breaks down the lactose into sugars. This chemical reaction is imperative…

To successfully co-reconstitute both enzymes into a single liposome it was necessary to first conduct detergent screening experiments for the reconstitution of each enzyme independently. To establish the most suitable detergent and detergent concentration, the magnitude of PMF generation along with enzymatic activity was measured for each reconstituted enzyme. Of the detergents tested, the zwitterionic surfactant CHAPS was found most suitable for…

the rate of reaction on Enzyme reaction Introduction: This experiment will investigate the effect of temperature on the rate of reaction of the enzyme catalase. The biological catalyst called enzymes increase the rate of reaction without themselves being chemically changed. Enzymes are globular proteins folded into a complex 3-dimensional shape that contains a special surface area called the active site where a specific substrate binds structurally and chemically. Enzymes speed up reactions…

of the experiment indicated that the rate of the reaction of enzyme increases whenever the concentration is high. Also, if the concentration is low, the reaction rate will be low too. Introduction Enzymes are proteins that make the chemical reactions faster by reducing the activation energy that is required to start the reaction, and they also act as catalyst. The substrate, which the enzyme acts upon, binds to the part of enzyme that is known as active site.…