Enzyme kinetics

temperature on the rate of liver enzyme activity is measured in this experiment to be able to visualize how temperature in the environment and in the body can manipulate the rate of enzyme activity. Enzymes are protein catalysts that lower the amount of activation energy required to start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is…

This lab looked at the different effects of an enzyme with concentration, pH, and temperature compared to the rate at which the enzyme works. The results showed a quick decrease when the temperature and pH has passed its most favorable conditions and or levels, but the concentration remains at the same levels throughout except pH6 and 40 degrees celsius. Both the pH and temperature had a steady increase in rate once the favorable conditions were being reached or are reached, but had a certain…

BIO LAB 1130.036 December 1, 2016 They call me DJ Enzyme because I 'm always breaking it down Abstract: Although protein function is thought to depend on the dynamics of the molecule and its environment contribute to catalytic mechanisms. The increase and decrease of temperatures affected the rate of reaction. In nature, organisms adjust the conditions of their enzymes to produce an optimum rate of reaction, where necessary, may have enzymes which are adapted to function well in extreme…

CYP3A4 and CYP3A5 enzymes. Metabolite profiling and Identification After incubating PTV with HLMs in the presence of a NADPH regenerating system, a metabolite (M2) of PTV was profiled, characterized, and identified by LC-MS/MS. A representative chromatograms are provided in Fig 2 (a) and (b). The LC-MS/MS analysis of the unchanged PTV and its metabolite produced informative and prominent product ions. The MS/MS spectrum of PTV, with a protonated molecular ion [M+H]+ at m/z…

As a result, lactose is a competitive inhibitor. In the presence of the inhibitor, the slope of the Lineweaver-Burk plot becomes the equation below. Using equation 7 along with the Km and the Vmax from series A, the Ki of Series B and C were calculated. The concentration of inhibitor for 50mM lactose was found to be 35714 μM using equation 1. Similar to the substrate concentration, the inhibitor concentration can be found using the same equation, but substituting in the inhibitor molar…

Potato Bubbles Introduction Our driving question for this lab was “How do changes in temperature and pH levels affect enzyme activity?” Throughout the duration of this lab, we explored enzymes and how environmental factors like pH and temperature affected enzyme activity. Enzymes are proteins that speed up chemical reactions. For this particular experiment, we studied the enzyme catalase, which is found in potatoes. Catalase reacts with hydrogen peroxide to break it down into its water and…

salicylate hydroxylase (11), anthranilate hydroxylase (12), melilolate hydroxylase (13). Generally, the catalytic cycles of these enzymes follow the reaction shown in Scheme 3. The reaction is composed of two parts: the reductive half-reaction and the oxidative half-reaction. Scheme 3 shows the catalytic cycle of p-hydroxybenzoate hydroxylase, the most extensively studied enzymes of the class, and also represents a general model of reaction mechanism for other single-component…

catalytic effect and specificity of enzymes 1.Introductions to enzymes Enzymes are proteins that can react with their specific substrates to achieve an highly effective catalyst reaction. .Almost all cellular activities of enzymes involved in the process are required to improve efficiency. Similar to other non-biological catalysts，enzymes provides another reaction route requires lower activation energy (Ea) to increase the number of particles that have a kinetic energy of not less than the…

Contreras, Ailly Lam, Aleksi Ska. Background information Enzymes are biological catalysts, which speed up the rate of chemical reactions. The pancreas produces a concentrated solution containing a mixture of enzymes – amylase, lipase and protease. In this experiment you will examine the effect of protease on casein, the protein found in milk. When the protease breaks down the casein, the opaque solution will become transparent. Theoretically, Enzyme activity increases with an increase in…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…