Enzyme kinetics

extracellular fraction from a stationary phase culture, to test the localization of the enzymes. For the protease assay, a specific soluble chromogenic substrate called azocasein was employed to determine presence of enzyme activity. The degradation of this substrate in trichloroacetic acid produces a colour change that can be registered using a spectrophotometer, and this change in absorbance over time is proportional to enzyme activity (Charney and Tomarelli, 1947). We measured the absorbance…

RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its constituent monosaccharrides, glucose and galactose. The amounts of proteins produced by this gene were observed to see what strain would yield the most. Each of the strains tested had a mutation that would…

Effects of Temperature and Substrate Concentration on Catalase Kristen Clark Biology 101 Lab Section 041 9/22/14 Brandon Stroup Abstract Catalase is an enzyme found in every living organism and is essential for cells. This lab explores how catalase reacts to four different temperatures and how the concentration percentage of substrate affects its reaction rate. The performance of catalase is dependent on many factors, so the purpose of the lab was to find the ideal concentration and…

called enzymes. These enzymes use various tactics to speed up the reaction. It may reduce the activation energy used to carry out the reaction, or it may break down large molecules so they will react faster. They may also form a new molecule by binding two old ones together (Castro). Enzymes are very particular, one specific enzyme can only speed up one specific reaction. there are factors that will affect the enzyme and it’s productivity, such as pH and substrate concentration. Every enzyme has…

Investigation was carried out to assess the effect of salt concentration on quality of sauerkraut prepared from mature cabbage head. Cabbage sample were chopped in small pieces and mixed with different salt concentrations ie., 2.0, 2.5, 3.0, 3.5 and 4.0 % and allowed to ferment for preparation of sauerkraut at ambient condition. Acidity of sauerkraut increased with increase in fermentation period and it reached maximum on 21 days after fermentation and thereafter it decreased in all the salt…

Protein Casein DCP & CE Hypothesis: Casein is made up of many individual amino acids. Each of them may have positively charge or negative charge depending on the pH of the milk. At certain pH value, positively charges and negatively charges of the casein will equal, so that the number of electrons equals to the number of protons. [1] Approximately pH 4.6 is the pH value that acid casein is precipitated and least soluble. In milk, which has a pH of about 6.6, the casein has a net negative…

Elizabeth Barron Winters July 9th, 2017 Case study 1 Atkins or Fadkins Part 1: Macronutrients and energy Question 1: Proteins: do most of the work in the cells, required for structure, function, and regulation of the bodys tissues and organs. They also transport nutrients and are catalyst. Examples of proteins are nuts, meats, and beans. Carbohydrates: source of energy for the body, simple and complex carbs. Examples are fruits, pasta, sugar, and, grain. Fats: store energy, aid in…

Discussion Graph 1 displays the reaction time (in minutes) of how long it took for the Alka-Seltzer tablet to completely dissolve per trial. Graph 2 displays the averages of the reaction time which showed the higher the water temperature is, the faster the reaction time would be. At 65⁰C the average time for the Alka-Seltzer to completely dissolve was 1 minute 4 seconds followed by 1 minute 20 seconds at 35⁰C and ending with the highest average at 2 minutes 1 second (1.61). The hypothesis…

Question: How does temperature affect the rate of osmosis in raisins? Background: Osmosis is the passage of a solvent through a semipermeable membrane from a less concentrated to a more concentrated solution until both solutions are of the same concentration We designed an experiment that would test if a raisins rate of osmosis is affected by the temperature of water it is placed in. The independent variable in this experiment was time which was 10 minute intervals. The dependent variable…

Aim: This experiment will investigate the effects of different concentrations of fertilizer on the growth of duckweed. Hypothesis: It is expected that the cup with the most amount of fertilizer (cup D) over the 14 days will have the most amount of growth compared to cup A, B and C. Apparatus: 4x polystyrene cups 20x duckweed fronds (5 in each cup) 1x 5ml medicine teaspoon 1x tweezers 1x syringe 1x marker fertilizer Method: 1. Label each cup as A, B, C or D with the marker 2. Using the…