Enzyme kinetics

substrate concentration (%N × 6.25), (ii) enzyme-substrate ratio (E/S) in % or in activity units per kg N × 6.25, (iii) pH, (iv) temperature, and (v) time (Adler-Nissen, 1984). Generally, there is an optimum combination of both pH and temerature, where the enzyme shows the highest activity. Physicochemical conditions of the hydrolysis reaction such as temperature, pH and enzyme/substrate ratio must be adjusted to optimize the activity of the proteolytic enzyme (Kim & Wijesekara, 2010; Santos,…

The Effect of Change in Temperature Conditions on Enzymes Bacillus licheniformis Amylase and Aspergillus oryzae Amylase Over Time ____________________________________ Roxanna Flores PID: 5199946 Lab Section U26 Group Members: Roudabyna Taleb Abstract This laboratory was performed in order to be able to determine the effect of temperature over time on bacterial amylase Bacillus licheniformis and fungal amylase Aspergillus oryzae. Bacterial amylase was introduced into four singular test tubes…

Acid in the stomach functions to kill bacteria, and aid digestion by solubilizing food. The acid is also important to establish the optimal pH (between 1.8 and 3.5) for the function of the digestive enzyme pepsin. Stimulation of acid secretion involves the translocation of H+/K+-ATPases to the apical membrane of the parietal cell. When the cell is resting (not stimulated), H+/K+-ATPases are located in vesicles inside the cell. When the cell is stimulated…

dilution of salt would have the gummy bear with the largest mass. This was because diffusion is the process where the molecules of both substances interact, with a constant kinetic energy. Therefore because the salt would increase the mass of the water, it would in result also increase the mass of the gummy bear because of the kinetic energy that interacts with both sets of molecules. In our planning stages we attempted to find a project idea which would vividly…

This experiment was centered on diffusion and osmosis. In order to see diffusion and osmosis in action it was required to test three different solutions on an egg: one with sugar, another with vinegar, and the last one with regular water. This would demonstrate certain interactions, and movements of the water; making the different concentrations of water visible. As stated there will be three solutions, this will help answer the question of how each type of solution will individually affect the…

The Effect of Lime Juice on a Gummy Bear’s Mass Lab Introduction Objective: In this lab we are going to learn the effect of lime juice on a gummy bear when it is soaked for 24 hours. We will see the effect is has on the mass when it is taken out. Information is Useful: This lab is an example of how cell transport works. The gummy bear is a cell in this case which is placed in a hypotonic solution that is the lime juice. Hyp0tonic solution is a greater concentration of the solute molecules…

Betacyanin pigments from beta vulgaris vacuoles were use in this experiment to determine the various effects of diffusion into its surrounding environment: 60%detergent, which is shown in the figures below. The four figures below are a set of data that were collected and analyzed the effects of time, salt concentration and ionic compositions on the rate of diffusion. Both figure one and two show a set of measurements between two different salt concentrations representing their absorbance versus…

Today, I concluded the experiment of last meal mix up. Through multiple test, I was able to decipher which stomach content belongs to what person. The first test was the brown paper bag test, which allows me to see if the stomach content contains lipids or not.. This involved taking a piece of bag paper and using a pencil to divide it into five sections. Next, I labeled each section with the number of the stomach contents. Then, I placed two drops of each sample in the appropriate section. After…

Effects of Different Concentrations of Caffeine on Cellular Respiration in a Saccharomyces Cerevisiae Cell Valentine Bedford Red Deer College- Red Deer Results The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL.…

The procedure of the experiment includes two parts: 1. Determination of the Freezing Point of Glacial Acetic Acid: to record the temperature of acetic acid every 30 seconds until it reach 300 seconds (5 minutes). 2. Determination of the Molar Mass of an Unknown Compound: to record the temperature of the mixture between unknown solid and glacial acetic acid. After that, put the ice-water mixture into the tube and record the temperature every 30 seconds. Pre lab questions: 1. Solute: dissolved…