Enzyme kinetics

and temperature on the caseinolytic activity of crude protease were done. Stability towards temperature and pH were also evaluated. The pH and temperature activity curves and other stability studies suggested the possible presence of one proteolytic enzyme in the latex of Vallaris solanacea. In the present study, separation and purification of the cysteine protease…

OBJECTIVE For the first week of the lab, the objectives were to isolate and test for the presence of major components of milk, including proteins, carbohydrates, fats, vitamins, and minerals and to determine the presence of the various components of milk in the various fractions using qualitative tests for these substances. For the second week of the lab, the there were three objectives that related to the procedure: to determine the calcium concentration of milk using the technique of…

Introduction The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols, hydroxamic acids, oximes, and sulfinic acids give positive results as well. The ferric chloride test can be used to detect metabolites in urine in case of inborn error of metabolism such as phenylketonuria. In addition,the Ferric Chloride test can be used to detect salicylates in urine after an aspirin overdose.1 Background…

The synthesis of Aa-g-poly(MAA) involve the optimization of various reaction conditions like initiator concentration, monomer concentration, crosslinker concentration, solvent concentration and pH. After the optimization of various reaction parameters the replications were carried-out in triplicates for the reproducibility of the results and statistical of the results obtained. It was performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the…

composite has been investigated. For that purpose, the PMMA samples with Al2O3 additive prepared with different percentages(0,15,25,35 and 45) wt.% and different thickness. The experimental results showed that the D.C electrical conductivity increased with increasing the alumina concentrations and temperature. Also the activation energy change with increasing of additional alumina. The dielectric constant, dielectric loss, A.C electrical conductivity are changed with change the concentration of…

Lipids are fatty acids and their derivatives, and substances related biosynthetically or functionally to these compounds. Lipids are diverse group of organic compounds including fats, oils, hormones, and certain components of membranes that are grouped together and mainly amphipathic. The variation in head groups and aliphatic chains allows the presence of more than 1000 different lipid species in any eukaryotic cell. Different types of lipid include Phospholipids, Sphingolipids and Cholesterol…

Burkholderia pseudomallei also called as pseudomonas pseudomallei is an intracellular gram negative bipolar staining aerobic motile shaped rod with a safety pin appearance when seen microscopically measuring 25 micro metre in length and 0.4 -0.8 micro metre in diameter . It is capable of self population using flagella. In vitro , it requires a temperature of 40 degree and grows in an acidic environment fermenting glucose and galactose . It produces both exotoxin and endotoxins. It causes…

3.1. Chemical composition and monosaccharide analysis Polysaccharide molecules are linked together by water molecules through hydrogen bonds in an aqueous solution. Adding ethanol into aqueous solution of polysaccharide initially dehydrate polysaccharides, enhance intra-molecular hydrogen bonding, cause conformational change and aggregation of polysaccharide molecules, and eventually leads to the precipitation of macromolecular chains, and the following addition of alcohol solvent gradually…

The purpose of this study was to investigate the effect of a system that automatically adjusting hydrogen peroxıde Colloıdal-Ag dose to reduce colonization and growth of heterotrophic bacteria in dental unit waterlines and wastewaters. Thirty dental units were assigned into three groups with respect to the protocol employed: Group 1: 10 units were selected for automatic administration. Group 2: 10 units were selected for manual administration and Group 3: 10 units served as controls. Water…

1 enzyme Enzymes are biological catalysts. A protein compound Energy can reduce the carbon reaction. Enzymes to accelerate specific types of reactions. And the kind of substance that reacts. 2.1.1 catalysis of enzymes E is a catalyst (enzyme). S is a precursor called chop, nitrates and substance P is a product. E + S ---------------> E S ---------------> E + P. Complexometry 2.1.2 Factors affecting the enzyme. 1) What type of controlled…