Enzyme kinetics

Table 1: Experimental data for standardization of iodine solution. Calculation of standardization factor Standardization factor = (mass of vitamin C titrated) / (Volume of iodine solution used) = (125g) / (41.3ml) = 3.03 mg vitamin C/ml iodine solution Experiment B: Vitamin C titration using fresh fruit sample to determine the content The tabulated experimental data for this experiment is shown below (Table 2). In this experiment, the amount of vitamin C was calculated by multiplying…

Results The results obtained were statistically analyzed using the Fishers exact test. Table 1: Comparison of microbial growth between different groups Sodium Hypochloride Total Fishers exact test Endovac Laser Control p-value No Growth 5(50.0%) 8(80.0%) 0 15(37.5%) <0.001* <103 5(50.0%) 2(20.0%) 0 7(17.5%) 103 – 108 0 0 0 8(20.0%) >108 0 0 10(100.0%) 10(25.0%) Total 10 10 10 40(100.0%) *p<0.05 statistically significant p>0.05 non significant, NS Amongst the 10…

Bessara of Fava Bean from Morocco Serving size: 1 Servings per Recipe: 4 Calories: 357 Cooking Time: 20 minutes Ingredients: 1. Garlic- 2 cloves, slightly crushed 2. Split Fava Beans- 2 cups 3. Vegetable oil- 2 tbsp. 4. Water-3 cups 5. Cumin Powder- 2 teaspoons 6. Tahini- 2 teaspoons 7. Harissa- 1 teaspoon 8. Lemon- squeezed juice and zest separately 9. Salt- 2 teaspoon 10. Paprika to taste 11. Olive oil- 1 tablespoon Nutritional Information: 1. Total Fat – 24.6g 2. Sodium- 335.6g 3. Total…

Phase Solubility results The phase-solubility diagram (figure 1) obtained was classified as AL type(20) because the apparent solubility of ISH increased linearly with β-CD concentration over the entire concentration range studies, thereby suggesting the formation of a water soluble complex. A slope value of less than one indicated that an inclusion complex in a molar stoichiometric ratio of 1:1 was formed between the drug and β-CD in solution. The stability constant (718.58 M–1), computed from…

Boris Kozarski Lab partner: Donovan Ortiz 9/23/16 Bio 118: A-65 GTA: Mian Li, UGTA: Shelby Schaffer Intro: Enzymes are important biological molecules that help catalyze numerous reactions in the body. They are an arrangement of proteins designed to serve very specific roles. Enzymes contain active sites that substrates bind to. The enzymes then help orient those substrates so they achieve ideal reaction conditions. As a result, substrates are broken down much faster than they would on their own…

ABSTRACT: This study was carried out to evaluate the effect of pretreatments prior different drying methods and temperatures on compositional quality of the dried mushroom. Oyster mushroom (Pleurotus ostreatus) was cultivated on rice substrate and collected samples were pretreated (steeped or blanched) by different solutions (0.1% sodium metabisulfite and 0.1 and 0.5% citric acid) and dried using oven at 50°C and 60°C, sun-drying at 30-35°C and microwave drying at 2.45 GHz until reached to…

introduce the basics of enzymes. The topic is important because they are in every living cell and because of enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the temperature, then the enzyme reaction time will also…

3.0 RESULTS AND DISCUSSION 3.1: Effect of Storage Conditions on Egg Weight Tables 1- 2 show the results of effect of storage conditions on eggs weight (chicken eggs and quail eggs). The weight of chicken eggs stored at ambient condition (control), moist sawdust, refrigerator and those that were dipped in oil reduced from 63.61-60.03g, 63.11-60.99g, 61.12-59.41g and 66.12-65.79g respectively. While the weight of quail eggs stored at ambient condition (control), moist sawdust, refrigerator and…

Characterization of the oxidized amylose and the linalool/oxidized amylose inclusion complex. The high water solubility of oxidized amylose can broaden the applications in water environment.14, 32 The Table 1 shows the properties of amylose and oxidized amylose. As expected, the solubility of oxidized amyloses in water is highly improved due to the introduced carboxyl and carbonyl groups. And it increases greatly with the increase of carboxyl content. The results suggest that due to the two-step…

Vmax value when compared to other 3 substrates (Table 1). Table 1: Substrate specificity of PPO enzyme Substrates Specific activity (units mg-1 protein) Km (µM) Vmax (abs min-1) Catechol 2300 4.12 X 10-3 3020 L-DOPA 430 16.40 X 10-3 990 L-Tyrosine 1930 6.12 X 10-3 2930 P-Cresol 2110 5.01 X 10-3 2983 Effect of Inhibitors The effects of various compounds on the PPO activity were provided in Tables 2. Enzyme activity was markedly…