Enzyme inhibitor

Discussion In this study, the Catechol enzyme was studied under the conditions of varying pH, temperature, substrate concentration, and enzyme concentration. In Figure 1, the data suggested that the trend was neither directly nor inversely proportional, but the highest activity rate was at 24°C. Most enzymes denatured at higher temperatures of approximately 40°C, which led to the inability to see any color change (Helms et al., 1998). At lower temperatures, the enzyme was somewhat efficient…

Introduction: The purpose of the tests (culture media, motility, enzymes, etc.) we preform on the numerous bacteria in lab is to show any biological or chemical characteristics of the bacteria that may help it survive or adapt in the wild, how it may be useful or hazardous to humans, and use as general knowledge to differentiate bacteria from each other. In the Culture Media test we can use solid and liquid media to grow multiple bacteria in lab. It can help us determine what processes the…

deprotonation of a serine residue, Zn(II) ion at M2 position then stimulates Ser102 to attack the phosphorus center of the substrate to initiate the leaving of an alcohol group and to form a phosphoseryl intermediate. This intermediate resembles E-Pi, an enzyme-phosphate complex, in Figure 2 and…

been caused by a liver dysfunction. According to Thepa (2007), serum bilirubin is elevated when there is an impairment in the excretion of conjugated bilirubin from hepatocytes of bile ducts. The results also showed elevation of two of the liver’s enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), these elevation could be caused by impaired liver function (Pratt et al, 2000). Mariana’s serum ALT levels is higher than the serum AST which could be caused by a form of…

ABSTRACT To examine the enzyme kinetics of chymotrypsin during the catalysis of the synthetic substrate p-nitrophenyl acetate (NPA). Initially, observe the magnitude of the initial burst with regards to the enzyme concentration. Secondly, the analysis of the steady state deacylation rate with regards to the pH of the reaction mixture. Chymotrypsin catalysis is a process that proceeds with two steps, an initial fast step involving the covalent modification of the enzyme to the substrate and the…

Experiment #6: Polarimetry Resolution of (∓)-Racemate: (∓)-ɑ-Phenylethylamine OBJECTIVE All chiral molecules have a mirror image of themselves. While they have the same physical properties, they interact with other chiral molecules completely different. Since there are many chiral molecules in organics, this can lead to the need to make optically pure products. If the chemist does not, it can lead to many problems including death. In this experiment, the separation of two enantiomers was…

Tetrahymena contractions with different solutions Purpose: How fast will the Tetrahymena contractile vacuole contract in different solutions? Introduction: In this experiment, the organism that is used are the Tetrahymena. The Tetrahymena has a contractile vacuole that protects them from absorbing too much water, causing cytolysis, in which a cell swells up and could result in explosion of the cell. The contractile vacuole expands when water enters it and contracts to release water to maintain…

and dibasic potassium. Phosphorus occurs naturally in every cell in the body and is used for repair and growth of cells and tissues. As an organic and inorganic phosphate, it has a significant biochemical function in the body creating metabolic and enzyme responses in almost all organs where it influences the steadiness of calcium levels, neutralizes acidic and basic ions in a solution and has a role in…

6. EXPERIMENAL WORK 6.1 Preliminary Work 6.1.1 Collection of Plant material The leaves of Jasminum grandiflorum was collected locally in the month of July locally from Bhopal, Madhya Pradesh, India. Herbarium file of plant part was prepared and authenticated by Dr. Zia Ul Hasan (Professor, Department of Botany), Safia College Bhopal and the specimen voucher no.assigned was 344/Bot/safia/15 6.1.2 Drying and Size Reduction of Plant Material The leaves of Jasminum grandiflorum was dried under…

Dulce De Leche caramelizad sauce Serving size: 1 jar Servings per Recipe: 60 Calories: 50 Cooking Time: 1 hour Ingredients: 1. Cold Water – 1 cup (250ml) 2. Condensed milk – 396 g, Sweetened 3. Baking soda - ½ teaspoon (3g) 4. Warm water - 3 tablespoons (45ml) Nutritional Information: 1. Total Fat – 1.5 g 2. Sodium- 20.0 mg 3. Total Carbs- 11.5 g 4. Fiber- 0.0 g 5. Cholesterol – 5.0 mg Directions: 1. To start with the recipe, set the timer of the Power Pressure Cooker to 40-60 minutes…