Enzyme assay

Because of the chemical inertness of MWCNTs, it is essential to create reactive groups on the surface for further modification [15]. The carboylic groups on the MWCNTs provide ideal anchoring point for the covalent immobilization of enzyme using EDC/NHS [15]. It has been demonstrated that sonicating MWCNTs in a mixture of acid solution opens the tube caps and forms carboxylic groups at the defect sites along the side walls [36]. Fig. 1 shows the FTIR spectra of MWCNTs and acid-treated MWCNTs.…

Enzymes speed up the rates of most biochemical reactions by helping reactants interact with each other. Since enzymes are highly specific for a specific reaction, they only catalyze one or a few types of reaction. One of important enzymes in human body and many other animals is amylase that hydrolyzes starch, a major part of human diet for many people in the world. There…

Fermentation Rate in Different Sugars Abstract: This experiment is designed by students after studying the process of cellular respiration and fermentation. It demonstrates students’ understanding over the materials and utilization of the lab setting. In this experiment, the primary focus will on the speed of fermentation in different substances (sugars) in the same condition (temperature, volume, amount of yeast). Each solution will be mix with a fix amount of yeast and put in fermentation tube…

The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

This bacteria had a positive catalase test meaning that it contains the enzyme catalase and was able to turn the hydrogen peroxide into water and oxygen and that this bacteria uses aerobic respiration. When plated on a mannitol/salt plate the bacteria was able to grow on the plate meaning that it has a resistance to high salt…

molecules are broken down into simpler compounds. (Butterworth, Ellis, Warren 2011). These polysaccharides made of glucose monomers are broken down into maltose, a disaccharide made of two glucose units. Further experimentations in 1833 found that an enzyme, later known as amylase, present in the…

Results Subjects Eight subjects completed this study with a mean BMI of 22.18 ±2.9 kg/m2( range, 19.5-28.15),aged 22 ±1.2 year (range 21-24 year), fasting glucose concentration of high carbohydrate meal is 4.9 ±0.071 mmol ( range, 4.6-5.2 mmol) and fasting glucose concentration of high protein meal is 5.0± 0.098 mmol (range, 4.7-5.5 mmol).There is no significant differences between the two test meals in the fasting values. Metabolic response Results for plasma glucose is depicted in the…

Specific environmental conditions may play a role in how enzyme activity is affected. We conducted an experiment to observe whether the change in temperature altered the reaction rate of an enzyme. The activity rate of our enzyme, catechol oxidase, was collected by analyzing the changes in absorption rate of the enzyme in a buffered catechol solution after being placed in four different temperatures. In which one solution remained out in room temperature being to be our negative control. Each of…

November 3rd, 2014 A Period Abstract: This report is a demonstration of different conditions an enzyme is exposed to and how the enzyme reacts in response. The conditions the enzyme was exposed to include: ideal conditions, the presence of a competitive substrate, denaturation for a short amount of time, and complete denaturation. The enzyme being observed was an imaginary one called “toothpickase”. This “enzyme” was to break toothpicks without any obstructions, the presence of paperclips as…