Enzyme assay

Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

of catechol oxidase used from a solanum tuberosum specimen Abstract The various effects of temperature and pH on the enzyme activity and action of catechol oxidase, an enzyme containing copper which oxidizes phenols such as catechol with the use of dioxygen, were examined throughout several trial procedures. The experiment was conducted to determine the optimal pH for an enzyme to carry out its action, as well as to determine…

aureus consist of a polyribitol-phosphate backbone of 11-40 repeating units(Xia10) decorated with three tailoring modifications: D-alanylation, a-O-GlcNAcylation and B-O-GlcNAcylation(Xia,Swaboda).Prior to this Brown’s work, most of the genes and enzymes involved in WTA synthesis and decoration had been identified, through genetic and in vitro reconstitution approaches(swoboda,xia). Although…

We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…

unable to do the same. S200T does not change the functional group of the residue, so it may still be able to act as a nucleophile. However, it may be limited without the charge carrying role of histidine. For all these reasons, it is probable that the enzyme has decreased or no function as a protease. In an animal, this may result in a decreased ability to digest and absorb…

Papillon-Lefevre syndrome (PLS) is a rare, autosomal recessive inherited, type IV palmoplantar keratoderma (Hart, et al., 2000). Its prevalence is approximately 4 cases per one million people with no sex or racial predominance (Sreeramulu, et al., 2015). Approximately 1 to 4 people per thousand are carriers of the disorder (Sreeramulu, et al., 2015). If both parents are carriers, there is only a 25% chance that their offspring will be affected. If both parents are affected with PLS, there is a…

Francisella tularensis is a bacterium that has been recognized to be an agent responsible for the disease known as tularemia. This disease, in essence, sends the immune system of the organism into a hyperactive state, sometimes referred to as hypercytokinemia due to the elevated levels of various cytokines, which if not treated properly could lead to extremely high fevers, inflammation of various tissues, and (if contracted through aerosol form) the death of the infected organism. Specifically,…

Then, using the same procedure as before, the solutions ' ultraviolet absorptions at 450 and 595 were determined and recorded in Table 4. As for the BCA assay, 100 microliters of each ovalbumin dilution were combined with 900 microliters of BCA, and the resulting solution were analyzed with a spectrophotometer in order to determine the uv absorbances at 560 nanometers (Data displayed in Table…

RESULTS Phytic Acid The concentration of phytic acid was determined spectrophotometrically in three tested plant materials of Gishta as shown in table 2. The concentrations of phytic acid were obtained from 5 g dried leafy material. All the leaf samples contained phytic acid (detection limit 0.025 mg/ml) in this study. This may have been due to the fact that there was a limitation in terms of the detection concentration since concentrations less than mg was not tested. Seed extracts was found…

Preparation of coating solutions Chitosan solution was prepared with 2 % (w/v) chitosan in 1% (v/v) acetic acid glacial. The solution was placed on a hot plate/magnetic stirrer for 6 h. The resultant chitosan-based coating solution was filtrated, through a Whatman filter paper No: 2, glycerol was added at 0.75 % (w/v), and stirred for more than 15 minutes. Two of the best concentrations, 60 and 90 mg mL−1 were prepared from freeze-dried IgY for S. putrefaciens and P. fluorescens respectively.…