Enzyme assay

Introduction Homeostasis is the state of a stable internal cell environment. If the cell is not in a state of homeostasis, then the cell will not be able to live and function properly. The plasma membrane is selectively permeable and controls what molecules enter and exit the cell. The plasma membrane keeps the environment inside the cell relatively stable which is the state of homeostasis. The goal of this investigation is to see if the size of the cell is affected when the cells are put in…

The purpose of this lab is to find the optimal pH of the enzyme peroxidase, which is found in plant and human cells as a byproduct of photorespiration, photosynthesis or cellular respiration. This is significant because an enzyme operating in nonoptimal pH could have negative effects on the cell, and could pose a health threat to the organism. Enzymes proteins that speed up chemical reactions by lowering the activation energy. They do this by acting as a template for substrate orientation,…

no molecules which fit the catalase enzyme and as such no reaction can take place. The reaction rate between the catalase and the hydrogen peroxide appeared to increase with the amount of available substrate (hydrogen peroxide). This is because catalase is capable of breaking down 40 million hydrogen peroxide molecules for every 1 catalase molecule and the more molecules of substrate present in the solution means that there are more molecules to fit the enzyme. The reaction results in more…

Introduction: All organisms depend on the action of enzymes to carry out the reactions of life. According to the text in Campbell Biology, enzymes are biological catalysts. These globular proteins speed the rate at which metabolic processes occur by lowering the activation energy or the energy barrier required to transform reactants to products. Each enzyme is specific, each containing active sites destined for a substrate. Substrates are the reactants or starting materials of chemical…

A group of biologists have developed a general model to explain growth and development in ectothermic organisms. Changes in environmental temperatures effect the rates of most biological reactions and biological processes in ectotherms. The rates of ontogenetic growth and development are no exceptions. Ectothermic animals develop faster at warmer temperatures and they usually mature at smaller body sizes. This phenomenon has been called the temperature size rule (Zuo et Al. 2011). The…

How can we differentiate acids and bases? How do we know if a substance is an acid or a base? There are different methods to discover whether a substance is an acid or a base. One of the methods is by using an acid-base indicator or by identifying hydrogen or hydroxide ions in a substance’s chemical formula. Certain materials such as a plastic template, red litmus paper, blue litmus paper, phenolphthalein, universal indicator, and a pH chart can be used to help us distinguish acids and bases.…

Enzymes are a biological catalyst. When a chemical reaction is taking place the enzyme works to lower the activation energy of a reaction. The activation energy is, the amount of energy that is required in order for a reaction to take place. Enzymes bind to reactant molecules. The enzymes hold these molecules in a way that makes the processes of the chemical bond-breaking and bond-forming take place more readily. A reaction can be either energy-releasing or energy-absorbing. Enzymes have no…

Question: How do different surface areas affect bacterial growth? Hypothesis: I think that the bacterial growth will be different in terms of size, shape, type and color. I think that different bacterias will grow on each quadrant because each surface of collection has unique bacterias. The unique bacterias will contribute to the fact that the size and color of them will also be different. I also predict that the growth of bacteria on the toilet seat (1D) will have the most colonies because it…

rate is described by the amount of product formed. Substances that increase that rate are called catalysts. Catalysts are often proteins called enzymes. Enzymes change the pathway of the reaction between the products and the product. However, enzymes don’t alter the starting or ending points. Enzymes are effective by reducing the activation energy. The enzyme tyrosinase is located in melanocytes. These are cells that produce the pigment melanin. Melanin gives skin, eyes, and hair their color.…

This experiment monitored the synthesis of uridine from the deamination of cytidine through separating the contents of the reaction mixture using HPLC (High-performance liquid chromatography) at various time intervals since the start of the reaction. Cytidine and uridine standards are ran and the output of the HPLC will show two peaks based on the retention times of each compound. Identified the peak with the retention time of 2.422 minutes is cytidine and uridine has a retention time of 2.716…