Enzyme assay

The procedure of the experiment includes two parts: 1. Determination of the Freezing Point of Glacial Acetic Acid: to record the temperature of acetic acid every 30 seconds until it reach 300 seconds (5 minutes). 2. Determination of the Molar Mass of an Unknown Compound: to record the temperature of the mixture between unknown solid and glacial acetic acid. After that, put the ice-water mixture into the tube and record the temperature every 30 seconds. Pre lab questions: 1. Solute: dissolved…

The objective of this experiment was to oxidize 9-fluorenol with bleach to form 9-fluorenone. This was done with three different reactions, each under different conditions. The reactions were evaluated using the TLC plate to see how far each reaction progressed. During the experiment reaction test tube 2 had the darkest yellow color out of the three reactions. On the TLC plate the starting material had one dot, with an Rf value of 0.22. Reaction 1 had two dots, with Rf values of 0.29 and…

In this lab, we took four separate onion bulbs, labeled them A,B,C, and D, and put them in a beaker filled with water. We recorded the growth in number of roots and their average length for days one, two, and five. Then we put Bulbs B, C, and D in beakers with different amounts of caffeine and left Bulb A in water. We then recorded the number of roots and their average length on days two, three, and four. After that, we took and onion root form Bulbs A, B, C, and D, stained them, and looked for…

Catherine Delker Honors Chemistry – Yellow Blue Beaker Experiment Lab Report 8/20/15 Blue Bottle Experiment Problem: The purpose of this lab was to determine the oxidation rate of the Methylene blue solution when tested with varying amounts of solution. Background: The Blue Bottle Experiment is a classic demonstration of a Reversible oxidation-reduction reaction with an alkaline solution of glucose that acts as a reducing agent. It is known that, “Methylene blue speeds up the reaction,…

LO 2.1 I understand that free energy, the portion of a biological system’s that can perform work when temperature and pressure are uniform throughout the system, is used in systems to cause change. I also understand that empirical data proves all organisms require a constant energy output to maintain organization, to grow, and to reproduce. (Campbell 119-123) LO 2.2 I understand that although free energy is always required in living systems in order to maintain organization, to grow, and to…

Like cars, humans need fuel for energy. While cars use gas for energy, humans use nutrients for energy such as proteins, lipids, and carbohydrates. Proteins, lipids, and carbohydrates are the macronutrients the body need to function smoothly and properly.Out of the three macronutrients, carbohydrates is the main source for energy, it is the body's main source of fuel, and the nutrient use for brain function and organ operation. Unlike proteins and lipids,carbohydrates is easily digested and can…

Phenylalanine hydroxylase is responsible for converting Phe to Tyr and requires the cofactor tetrahydrobiopterin (BH4), molecular oxygen, and iron for its action (Blau et al., 2010). The reductant, BH4 is maintained in the reduced state by the NADH-dependent enzyme dihydropteridine reductase. Decreased PAH activity results in elevated Phe concentrations in blood and body tissue. As a result, approximately 75% of untreated patients experience severe neurological dysfunction (Brumm and Grant,…

bath. We then repeated the same procedure using starch solution instead of maltose. When we did the test for digestion of starch. First, using separate medicine droppers, I transferred 3 ml of starch solution to a small beaker, then added a fresh enzyme solution consisting of a pinch of amylase in a 3ml of water and mixed thoroughly with a glass rod. Secondly, I waited for 1 min…

Simultaneous hydrolysis and fermentation (SHF) of sweet sorghum juice is a multi-step process and interactions between enzymes inside the yeast and the complex substrate. In addition, SHF also undergoesmechanisms by the product and other components inside the substrate. The process, interactions, and as well as inhibition mechanism of SHF are not fully understood. A modified kinetics model based on those reported by Philippidis [24] and Shadbahr [17] were used in this study to quantify the…

Enzyme catalysis can be a very confusing and long process, but in easier terms can just be explained by the fact that enzymes break down molecules into smaller pieces. The objective of this lab is to observe how quickly the enzyme catalase breaks down H2O2, and what long periods of contact between the two do to that process. We used seven different cups for seven different lengths of time, and in every cup we mixed 10 mL of 1.5% H2O2, 1 mL of distilled water, and 1 mL of catalase. We then…