Enzyme assay

Abstract The purpose of this investigation is to determine whether the temperature can affect the reaction rate of enzyme activity. The enzyme activity was measured by five different temperatures, separated in tubes which later got measured by their height of foam after 10 minutes. Freshly-prepared potato juice was used as the enzyme for catalase. Since temperature is measured by the kinetic energy of molecules, the higher the temperature, the more movement of molecules. The lowest temperature…

The Effect of Change in Temperature Conditions on Enzymes Bacillus licheniformis Amylase and Aspergillus oryzae Amylase Over Time ____________________________________ Roxanna Flores PID: 5199946 Lab Section U26 Group Members: Roudabyna Taleb Abstract This laboratory was performed in order to be able to determine the effect of temperature over time on bacterial amylase Bacillus licheniformis and fungal amylase Aspergillus oryzae. Bacterial amylase was introduced into four singular test tubes…

Acid in the stomach functions to kill bacteria, and aid digestion by solubilizing food. The acid is also important to establish the optimal pH (between 1.8 and 3.5) for the function of the digestive enzyme pepsin. Stimulation of acid secretion involves the translocation of H+/K+-ATPases to the apical membrane of the parietal cell. When the cell is resting (not stimulated), H+/K+-ATPases are located in vesicles inside the cell. When the cell is stimulated…

For our diffusion experiment our group decided to work with salt water and gummy bears. Our experiment tested the weight of the gummy bears per salt dilutions. Our tests included 1g 2.5g 5g, 1.5g, and 10g of salt diluted with water. We knew that the higher the concentration of salt diluted into the water the heavier the diffused gummy bear would be, therefore we measure weight as our numerical data. My hypothesis was that the 10g dilution of salt would have the gummy bear with the largest…

count-1025 Background research Enzymes are catalysts which speed up reactions in living organisms. Enzymes function by decreasing the activation energy for a reaction. Amylase is an enzyme found in human saliva and catalyses the hydrolysis of starch .Amylase like all other proteins have secondary and tertiary structures which are affected by temperature, pH and other factors. Due to the fact that enzyme function is related to the structure of any enzyme; enzymes are heavily affected by…

This experiment was centered on diffusion and osmosis. In order to see diffusion and osmosis in action it was required to test three different solutions on an egg: one with sugar, another with vinegar, and the last one with regular water. This would demonstrate certain interactions, and movements of the water; making the different concentrations of water visible. As stated there will be three solutions, this will help answer the question of how each type of solution will individually affect the…

The Effect of Lime Juice on a Gummy Bear’s Mass Lab Introduction Objective: In this lab we are going to learn the effect of lime juice on a gummy bear when it is soaked for 24 hours. We will see the effect is has on the mass when it is taken out. Information is Useful: This lab is an example of how cell transport works. The gummy bear is a cell in this case which is placed in a hypotonic solution that is the lime juice. Hyp0tonic solution is a greater concentration of the solute molecules…

Betacyanin pigments from beta vulgaris vacuoles were use in this experiment to determine the various effects of diffusion into its surrounding environment: 60%detergent, which is shown in the figures below. The four figures below are a set of data that were collected and analyzed the effects of time, salt concentration and ionic compositions on the rate of diffusion. Both figure one and two show a set of measurements between two different salt concentrations representing their absorbance versus…

Today, I concluded the experiment of last meal mix up. Through multiple test, I was able to decipher which stomach content belongs to what person. The first test was the brown paper bag test, which allows me to see if the stomach content contains lipids or not.. This involved taking a piece of bag paper and using a pencil to divide it into five sections. Next, I labeled each section with the number of the stomach contents. Then, I placed two drops of each sample in the appropriate section. After…

Effects of Different Concentrations of Caffeine on Cellular Respiration in a Saccharomyces Cerevisiae Cell Valentine Bedford Red Deer College- Red Deer Results The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL.…