Enzyme assay

Gasmalla et al Trop J Pharm Res, January 2014;13 (1): 61 Tropical Journal of Pharmaceutical Research January 2014; 13 (1): 61-65 ISSN: 1596-5996 (print); 1596-9827 (electronic) © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v13i1.9 Original Research Article Nutritional Composition of Stevia rebaudiana Bertoni Leaf: Effect of Drying Method Mohammed Abdalbasit…

Results and Discussion A. Diffusion of selected plant pigments In conducting the diffusion of 4 different kinds of treatments (distilled water, distilled water placed in boiling bath, vegetable oil, heated vegetable oil), the researchers made use of the atsuete and sugar beets. The different kinds of treatments determined how the temperature will affect the color intensity of the atsuete and sugar beets. There were 4 test tubes used in each atsuete and sugar beets with each having a…

FTIR analysis of the stem bark extracts of Cassia fistula has absorption bands and the wave numbers (cm-1) of the prominent peaks obtained were described in Table 2. The peak at a frequency of 3463 cm-1, 1618 cm-1, 1448.3 cm-1 and 1241.9 cm-1 were strong while the others vary from medium to weak. Discussion: Phytochemical studies revealed the presence of secondary metabolites like tannins, flavonoid, carbohydrate, saponins, glycosides and phenol in the leaf extract while tannins, flavonoids,…

Docking: In molecular modeling field, docking is a method which assumes the desired orientation of one molecule to a second molecule when bound to each other, to form a stable complex. Knowledge of the orientations can be used to assume the strength of association or binding affinity between the two molecules. The associations between biologically relevant molecules such as proteins, nucleic acids, carbohydrates, and lipids play a key role in signal transduction. Thus, the relative alignment of…

protein structure prediction is one among the foremost necessary goals pursued by bioinformatics and theoretical chemistry; it's extremely necessary in medication (for example, in drug design) and biotechnology (for example, within the style of novel enzymes). each 2 years, the performance of current ways is assessed within the CASP experiment (Critical Assessment of Techniques for protein Structure…

Abstract: Catalase is an iron porphyrin enzyme present in high concentrations in humans and animals liver tissues. It is a peroxisomal enzyme that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate. Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site…

multi copper oxidases (Mate and Alcalde, 2016a).They catalyze the oxidation of compounds that are coupled with the four-electron reduction of "O" _"2" "to" "H" _"2" "O" . They are regarded as eco-friendly enzymes. Laccases have been first discovered in 1883, which makes it one of the oldest enzymes ever observed (Kunamneni et al., 2008).Laccases are found in a diversity of fungi, bacteria and higher plant species (Santhanam et al., 2011). These laccases are involved in a degree of…

The role of oxysterols in human body and disease 1. Introduction Oxysterols are 27-carbon derivatives of cholesterol created by spontaneous or enzymatic oxidation (Figure 1, (Brown and Jessup, 2009)). The oxidation of cholesterol occurs either on the side chain or on the sterol core. Side chain oxidation generates, e.g., 24-hydroxycholesterol (24-HC), 25-hydroxycholesterol (25-HC) or 27-hydroxycholesterol (27-HC) while the core oxidation gives rise to ring oxysterols, mostly ring-B oxysterols,…

Introduction The purpose of this lab was to identify the lipids in an egg yolk and to identify the amount of lipids in milk [1]. To extract the lipids from the egg yolk, the egg yolk needed to be combined with an organic solvent such as a mixture of CHCl3/MeOH. An organic solvent was needed to carry the extracted lipids up the TLC plate because the plate was polar, due to the presence of silica gel (silanol groups). To compare relatively similar lipids to the ones found in the egg yolk, the egg…

substrate concentration (%N × 6.25), (ii) enzyme-substrate ratio (E/S) in % or in activity units per kg N × 6.25, (iii) pH, (iv) temperature, and (v) time (Adler-Nissen, 1984). Generally, there is an optimum combination of both pH and temerature, where the enzyme shows the highest activity. Physicochemical conditions of the hydrolysis reaction such as temperature, pH and enzyme/substrate ratio must be adjusted to optimize the activity of the proteolytic enzyme (Kim & Wijesekara, 2010; Santos,…